CDK12 抗体 (AA 1147-1439)
Quick Overview for CDK12 抗体 (AA 1147-1439) (ABIN7599764)
抗原
See all CDK12 (CRKRS) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1147-1439
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原理
- Anti-CDK12 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-CDK12 Antibody Picoband® (ABIN7599764). Tested in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human CDK12 recombinant protein (Position: Q1147-N1439).
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亚型
- IgG
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anti-Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS) (AA 80-290) antibody
CRKRS 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS) (AA 1281-1380) antibodyCRKRS 适用: 人 ELISA, WB, IF 宿主: 小鼠 Monoclonal 1H4 unconjugated
anti-Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS) (AA 1281-1380) antibodyCRKRS 适用: 人 ELISA, WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 3C1 unconjugated
anti-Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS) antibodyCRKRS 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS) (Internal Region) antibodyCRKRS 适用: 人, 犬, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS) (AA 1281-1380) antibodyCRKRS 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS) (AA 1309-1490) antibodyCRKRS 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS) (C-Term) antibodyCRKRS 适用: 人, 大鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Blazek, D., Kohoutek, J., Bartholomeeusen, K., Johansen, E., Hulinkova, P., Luo, Z., Cimermancic, P., Ule, J., Peterlin, B. M. The cyclin K/Cdk12 complex maintains genomic stability via regulation of expression of DNA damage response genes. Genes Dev. 25: 2158-2172, 2011. 2. Cheng, S.-W. G., Kuzyk, M. A., Moradian, A., Ichu, T.-A., Chang, V. C.-D., Tien, J. F., Vollett, S. E., Griffith, M., Marra, M. A., Morin, G. B. Interaction of cyclin-dependent kinase 12/CrkRS with cyclin K1 is required for the phosphorylation of the C-terminal domain of RNA polymerase II. Molec. Cell. Biol. 32: 4691-4704, 2012. 3. Dubbury, S. J., Boutz, P. L., Sharp, P. A. CDK12 regulates DNA repair genes by suppressing intronic polyadenylation. Nature 564: 141-145, 2018. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- CDK12 (CRKRS) (Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS))
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别名
- CDK12
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背景
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Synonyms: Calpain-2 catalytic subunit,3.4.22.53,Calcium-activated neutral proteinase 2,CANP 2,Calpain M-type,Calpain large polypeptide L2,Calpain-2 large subunit,Millimolar-calpain,M-calpain,CAPN2,CANPL2,
Tissue Specificity: Ubiquitous.
Background: CDK12 cyclin-dependent kinase 12 is a protein kinase that in humans is encoded by the CDK12 gene. This enzyme is a member of cyclin-dependent kinase protein family. CDK12 forms protein complexes with cyclin K (CCNK). The CDK12-CCNK complex acts at the level of RNA polymerase II (RNAPII) to regulate expression of long complex genes, several of which have roles in DNA maintenance and repair.
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分子量
- 205 kDa
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基因ID
- 51755
抗原
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