NFS1 抗体 (AA 107-356)
Quick Overview for NFS1 抗体 (AA 107-356) (ABIN7599676)
抗原
See all NFS1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 107-356
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原理
- Anti-NFS1 Antibody Picoband®
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产品特性
- Anti-NFS1 Antibody Picoband® (ABIN7599676). Tested in WB, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human NFS1 recombinant protein (Position: R107-L356). Human NFS1 shares 96.4% and 96% amino acid (aa) sequence identity with mouse and rat NFS1, respectively.
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anti-NFS1, Cysteine Desulfurase (NFS1) (AA 208-457) antibody
NFS1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NFS1, Cysteine Desulfurase (NFS1) (AA 208-457) antibodyNFS1 适用: 人 WB, IHC, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-NFS1, Cysteine Desulfurase (NFS1) antibodyNFS1 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-NFS1, Cysteine Desulfurase (NFS1) antibodyNFS1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NFS1, Cysteine Desulfurase (NFS1) (AA 1-457) antibodyNFS1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-NFS1, Cysteine Desulfurase (NFS1) antibodyNFS1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-NFS1, Cysteine Desulfurase (NFS1) (AA 29-138) antibodyNFS1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D5 unconjugated
anti-NFS1, Cysteine Desulfurase (NFS1) (AA 151-200) antibodyNFS1 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-NFS1, Cysteine Desulfurase (NFS1) antibodyNFS1 适用: 人 WB, DB 宿主: 小鼠 Monoclonal 2635E1a unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Alvarez, S. W., Sviderskiy, V. O., Terzi, E. M., Papagiannakopoulos, T., Moreira, A. L., Adams, S., Sabatini, D. M., Birsoy, K., Possemato, R. NFS1 undergoes positive selection in lung tumours and protects cells from ferroptosis. Nature 551: 639-643, 2017. Note: Erratum: Nature 609: E12, 2022. 2. Biederbick, A., Stehling, O., Rosser, R., Niggemeyer, B., Nakai, Y., Elsasser, H.-P., Lill, R. Role of human mitochondrial Nfs1 in cytosolic iron-sulfur protein biogenesis and iron regulation. Molec. Cell. Biol. 26: 5675-5687, 2006. 3. Farhan, S. M. K., Wang, J., Robinson, J. F., Lahiry P., Siu, V. M., Prasad, C., Kronick, J. B., Ramsay, D. A., Rupar C. A., Hegele, R. A. Exome sequencing identifies NFS1 deficiency in a novel Fe-S cluster disease, infantile mitochondrial complex II/III deficiency. Molec. Genet. Genomic Med. 2: 73-80, 2014. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- NFS1 (NFS1, Cysteine Desulfurase (NFS1))
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别名
- NFS1
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背景
- Cysteine desulfurase, mitochondrial is an enzyme that in humans is encoded by the NFS1 gene. Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH . The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described.
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分子量
- 45 kDa
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基因ID
- 9054
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UniProt
- Q9Y697
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途径
- Transition Metal Ion Homeostasis
抗原
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