14-3-3 gamma 抗体 (AA 107-138)
Quick Overview for 14-3-3 gamma 抗体 (AA 107-138) (ABIN7599673)
抗原
See all 14-3-3 gamma (YWHAG1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 107-138
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原理
- Anti-14-3-3 gamma/YWHAG Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-14-3-3 gamma/YWHAG Antibody (ABIN7599673). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human 14-3-3 gamma/YWHAG recombinant protein (Position: Y107-A138).
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亚型
- IgG
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anti-14-3-3 gamma (YWHAG1) (AA 70-169) antibody
YWHAG1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6A10 unconjugated
anti-14-3-3 gamma (YWHAG1) (AA 113-194) antibodyYWHAG1 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 gamma (YWHAG1) (Internal Region) antibodyYWHAG1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 gamma (YWHAG1) (AA 136-164) antibodyYWHAG1 适用: 人, 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB20871 unconjugated
anti-14-3-3 gamma (YWHAG1) antibodyYWHAG1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 gamma (YWHAG1) (AA 1-247) antibodyYWHAG1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B4 unconjugated
anti-14-3-3 gamma (YWHAG1) (AA 1-189) antibodyYWHAG1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 gamma (YWHAG1) antibodyYWHAG1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-14-3-3 gamma (YWHAG1) (AA 51-100) antibodyYWHAG1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 gamma (YWHAG1) antibodyYWHAG1 适用: 人, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Autieri, M. V., Carbone, C. J. 14-3-3-Gamma interacts with and is phosphorylated by multiple protein kinase C isoforms in PDGF-stimulated human vascular smooth muscle cells. DNA Cell Biol. 18: 555-564, 1999. 2. Cornell, B., Wachi, T., Zhukarev, V., Toyo-oka, K. Overexpression of the 14-3-3-gamma protein in embryonic mice results in neuronal migration delay in the developing cerebral cortex. Neurosci. Lett. 628: 40-46, 2016. 3. Horie, M., Suzuki, M., Takahashi, E., Tanigami, A. Cloning, expression, and chromosomal mapping of the human 14-3-3gamma gene (YWHAG) to 7q11.23. Genomics 60: 241-243, 1999. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- 14-3-3 gamma (YWHAG1)
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别名
- YWHAG
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背景
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Synonyms: Prostaglandin E synthase 3, Cytosolic prostaglandin E2 synthase, Cpges, Hsp90 co-chaperone, Progesterone receptor complex p23, Telomerase-binding protein p23, PTGES3, P23, TEBP
Tissue Specificity: Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.
Background: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100 % identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
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分子量
- 28 kDa
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基因ID
- 7532
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UniProt
- P61981
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途径
- Myometrial Relaxation and Contraction, M Phase
抗原
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