Phospholipase C delta 3 抗体 (AA 10-702)
Quick Overview for Phospholipase C delta 3 抗体 (AA 10-702) (ABIN7599611)
抗原
See all Phospholipase C delta 3 (PLCd3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 10-702
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原理
- Anti-PLCD3 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PLCD3 Antibody Picoband® (ABIN7599611). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PLCD3 recombinant protein (Position: R10-Q702).
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亚型
- IgG
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anti-Phospholipase C delta 3 (PLCd3) (AA 261-274) antibody
Verified PLCd3 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Phospholipase C delta 3 (PLCd3) (AA 528-789) antibodyPLCd3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ghosh, S., Pawelczyk, T., Lowenstein, J. M. Phospholipase C isoforms delta-1 and delta-3 from human fibroblasts: high-yield expression in Escherichia coli, simple purification, and properties. Protein Expr. Purif. 9: 262-278, 1997. 2. Kim, H., Suh, P. G., Ryu, S. H., Park, S. H. Assignment of the human PLC-delta-3 gene (PLCD3) to human chromosome band 17q21 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 87: 209-210, 1999. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- Phospholipase C delta 3 (PLCd3)
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别名
- PLCD3
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背景
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Synonyms: PR domain zinc finger protein 5, PR domain-containing protein 5, PRDM5, PFM2
Tissue Specificity: Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues.
Background: 1-Phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-3 is an enzyme that in humans is encoded by the PLCD3 gene. This gene encodes a member of the phospholipase C family, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate the second messengers diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Diacylglycerol and IP3 mediate a variety of cellular responses to extracellular stimuli by inducing protein kinase C and increasing cytosolic Ca(2+) concentrations. This enzyme localizes to the plasma membrane and requires calcium for activation. Its activity is inhibited by spermine, sphingosine, and several phospholipids.
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分子量
- 94 kDa
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基因ID
- 113026
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UniProt
- Q8N3E9
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途径
- WNT signaling
抗原
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