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ACAT1 抗体 (AA 10-404)

This anti-ACAT1 antibody is a 兔 多克隆 antibody detecting ACAT1 in WB, IHC, ELISA, IF, ICC 和 FACS. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN7599600
发货至: 中国

Quick Overview for ACAT1 抗体 (AA 10-404) (ABIN7599600)

抗原

See all ACAT1 抗体
ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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  • 10
多克隆

标记

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This ACAT1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
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    AA 10-404

    原理

    Anti-ACAT1 Antibody Picoband®

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-ACAT1 Antibody Picoband® (ABIN7599600). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human ACAT1 recombinant protein (Position: S10-Q404).

    亚型

    IgG
  • 应用备注

    Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Daum, R. S., Scriver, C. R., Mamer, O. A., Delvin, E., Lamm, P. H., Goldman, H. An inherited disorder of isoleucine catabolism causing accumulation of alpha-methylacetoacetate and alpha-methyl-beta-hydroxybutyrate and intermittent metabolic acidosis. Pediat. Res. 7: 149-160, 1973. 2. Fukao, T., Matsuo, N., Zhang, G. X., Urasawa, R., Kubo, T., Kohno, Y., Kondo, N. Single base substitutions at the initiator codon in the mitochondrial acetoacetyl-CoA thiolase (ACAT1/T2) gene result in production of varying amounts of wild-type T2 polypeptide. Hum. Mutat. 21: 587-592, 2003. 3. Fukao, T., Nakamura, H., Nakamura, K., Perez-Cerda, C., Baldellou, A., Barrionuevo, C. R., Castello, F. G., Kohno, Y., Ugarte, M., Kondo, N. Characterization of six mutations in five Spanish patients with mitochondrial acetoacetyl-CoA thiolase deficiency: effects of amino acid substitutions on tertiary structure. Molec. Genet. Metab. 75: 235-243, 2002.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    储存条件

    4 °C,-20 °C

    储存方法

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • 抗原

    ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

    别名

    ACAT1

    背景

    Synonyms: Bifunctional epoxide hydrolase 2,Cytosolic epoxide hydrolase 2,CEH,3.3.2.10,Epoxide hydratase,Soluble epoxide hydrolase,SEH,Lipid-phosphate phosphatase,3.1.3.76,EPHX2,

    Tissue Specificity: Ubiquitous. A high level expression is seen in secretory tissues.

    Background: Acetyl-CoA acetyltransferase, mitochondrial, also known as acetoacetyl-CoA thiolase, is an enzyme that in humans is encoded by the ACAT1 (Acetyl-Coenzyme A acetyltransferase 1) gene. This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

    分子量

    40 kDa

    基因ID

    38

    UniProt

    P24752
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