LIG4 抗体 (AA 1-876)
Quick Overview for LIG4 抗体 (AA 1-876) (ABIN7599567)
抗原
See all LIG4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-876
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原理
- Anti-DNA Ligase IV/LIG4 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-DNA Ligase IV/LIG4 Antibody Picoband® (ABIN7599567). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human DNA Ligase IV/LIG4 recombinant protein (Position: M1-K876).
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亚型
- IgG
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anti-Ligase IV, DNA, ATP-Dependent (LIG4) (C-Term) antibody
LIG4 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (AA 612-911) antibodyLIG4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (N-Term) antibodyLIG4 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (AA 275-324) antibodyLIG4 适用: 人, 马, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) antibodyKO Validated LIG4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (AA 802-911) antibodyLIG4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D2 unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (N-Term) antibodyLIG4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (Center) antibodyLIG4 适用: 人, Cow, 犬 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) antibodyLIG4 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (AA 1-911) antibodyLIG4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ben-Omran, T. I., Cerosaletti, K., Concannon, P., Weitzman, S., Nezarati, M. M. A patient with mutations in DNA ligase IV: clinical features and overlap with Nijmegen breakage syndrome. Am. J. Med. Genet. 137A: 283-287, 2005. 2. Frank, K. M., Sekiguchi, J. M., Seidl, K. J., Swat, W., Rathbun, G. A., Cheng, H.-L., Davidson, L., Kangaloo, L., Alt, F. W. Late embryonic lethality and impaired V(D)J recombination in mice lacking DNA ligase IV. Nature 396: 173-177, 1998. 3. Frank, K. M., Sharpless, N. E., Gao, Y., Sekiguchi, J. M., Ferguson, D. O., Zhu, C., Manis, J. P., Horner, J., DePinho, R. A., Alt, F. W. DNA ligase IV deficiency in mice leads to defective neurogenesis and embryonic lethality via the p53 pathway. Molec. Cell 5: 993-1002, 2000. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))
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别名
- LIG4
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背景
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Synonyms: Cell surface glycoprotein MUC18, Gicerin, Melanoma cell adhesion molecule, Melanoma-associated antigen MUC18, CD146, Mcam, Muc18
Tissue Specificity: Detected in melanoma cell lines.
Background: DNA ligase 4 is an enzyme that in humans is encoded by the LIG4 gene. The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
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分子量
- 104 kDa
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基因ID
- 3981
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UniProt
- P49917
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途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
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