PICALM 抗体 (AA 1-652)
Quick Overview for PICALM 抗体 (AA 1-652) (ABIN7599516)
抗原
See all PICALM 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-652
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原理
- Anti-PICALM Picoband® Antibody
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PICALM Picoband® Antibody (ABIN7599516). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PICALM recombinant protein (Position: M1-M652).
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亚型
- IgG
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anti-Phosphatidylinositol Binding clathrin Assembly Protein (PICALM) (AA 517-624) antibody
PICALM 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylinositol Binding clathrin Assembly Protein (PICALM) (AA 420-646) antibodyPICALM 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylinositol Binding clathrin Assembly Protein (PICALM) (AA 251-350) antibodyPICALM 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylinositol Binding clathrin Assembly Protein (PICALM) (AA 385-415) antibodyPICALM 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal RB53756 unconjugated
anti-Phosphatidylinositol Binding clathrin Assembly Protein (PICALM) (AA 512-543) antibodyPICALM 适用: 人 WB 宿主: 兔 Polyclonal RB54131 unconjugated
anti-Phosphatidylinositol Binding clathrin Assembly Protein (PICALM) (AA 1-307) antibodyPICALM 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylinositol Binding clathrin Assembly Protein (PICALM) (AA 1-610) antibodyPICALM 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Phosphatidylinositol Binding clathrin Assembly Protein (PICALM) (AA 438-487) antibodyPICALM 适用: 人, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼, Bat, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylinositol Binding clathrin Assembly Protein (PICALM) (AA 277-326) antibodyPICALM 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, Bat, 猴, Pig, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylinositol Binding clathrin Assembly Protein (PICALM) (AA 290-460) antibodyPICALM 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Morris, S. A., Schroder, S., Plessmann, U., Weber, K., Ungewickell, E. Clathrin assembly protein AP180: primary structure, domain organization and identification of a clathrin binding site. EMBO J. 12: 667-675, 1993. 2. Stern, J. A., White, S. N., Lehmkuhl, L. B., Reina-Doreste, Y., Ferguson, J. L., Nascone-Yoder, N. M., Meurs, K. M. A single codon insertion in PICALM is associated with development of familial subvalvular aortic stenosis in Newfoundland dogs. Hum. Genet. 133: 1139-1148, 2014. 3. Treusch, S., Hamamichi, S., Goodman, J. L., Matlack, K. E. S., Chung, C. Y., Baru, V., Shulman, J. M., Parrado, A., Bevis, B. J., Valastyan, J. S., Han, H., Lindhagen-Persson, M., and 9 others. Functional links between amyloid-beta toxicity, endocytic trafficking, and Alzheimer's disease risk factors in yeast. Science 334: 1241-1245, 2011. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))
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别名
- PICALM
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背景
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Synonyms: Phosphatidylinositol-binding clathrin assembly protein, Clathrin assembly lymphoid myeloid leukemia protein, PICALM, CALM
Tissue Specificity: In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 78 % of tested tumors (at protein level). Ubiquitously expressed in non-cancerous tissues.
Background: Phosphatidylinositol binding clathrin assembly protein, also known as PICALM, is a protein which in humans is encoded by the PICALM gene. It is mapped to 11q14.2. This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t (10,11) (p13,q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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分子量
- 71 kDa
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基因ID
- 8301
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UniProt
- Q13492
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途径
- Synaptic Membrane
抗原
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