PCK1 抗体 (AA 1-622)
Quick Overview for PCK1 抗体 (AA 1-622) (ABIN7599502)
抗原
See all PCK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-622
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原理
- Anti-PCK1/PEPC Antibody
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PCK1/PEPC Antibody (ABIN7599502). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PCK1/PEPC recombinant protein (Position: M1-M622).
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亚型
- IgG
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anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (C-Term) antibody
PCK1 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 592-622), (C-Term) antibodyPCK1 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF 宿主: 兔 Polyclonal RB03646 unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) antibodyPCK1 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 3E4 unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (Middle Region) antibodyPCK1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 马, Pig, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 312-563) antibodyPCK1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (Middle Region) antibodyPCK1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 马, Pig, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (C-Term) antibodyPCK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 330-379) antibodyPCK1 适用: 人, 小鼠, 大鼠, Cow, 兔, 马, 猴, 小鸡, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 311-558) antibodyPCK1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) antibodyPCK1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
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别名
- PCK1
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背景
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Synonyms: Tripartite motif-containing protein 6, RING finger protein 89, RING-type E3 ubiquitin transferase TRIM6, TRIM6, RNF89
Tissue Specificity: Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. .
Background: This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized.
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分子量
- 69 kDa
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基因ID
- 5105
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UniProt
- P35558
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途径
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
抗原
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