SKIL 抗体 (AA 1-504)
Quick Overview for SKIL 抗体 (AA 1-504) (ABIN7599455)
抗原
See all SKIL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-504
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原理
- Anti-SnoN/SNO/SKIL Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-SnoN/SNO/SKIL Antibody Picoband® (ABIN7599455). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human SnoN/SNO/SKIL recombinant protein (Position: M1-V504).
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亚型
- IgG
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anti-SKI-Like Oncogene (SKIL) (AA 307-684) antibody
SKIL 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3E2 unconjugated
anti-SKI-Like Oncogene (SKIL) (AA 359-579) antibodySKIL 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SKI-Like Oncogene (SKIL) (AA 236-313) antibodySKIL 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C12 unconjugated
anti-SKI-Like Oncogene (SKIL) (C-Term) antibodySKIL 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SKI-Like Oncogene (SKIL) (AA 301-400) antibodySKIL 适用: 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-SKI-Like Oncogene (SKIL) (C-Term) antibodySKIL 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-SKI-Like Oncogene (SKIL) (C-Term) antibodySKIL 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-SKI-Like Oncogene (SKIL) (AA 307-684) antibodySKIL 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-SKI-Like Oncogene (SKIL) (AA 542-591) antibodySKIL 适用: 人, 大鼠, Cow, 豚鼠, Pig, 猴, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated
anti-SKI-Like Oncogene (SKIL) (C-Term) antibodySKIL 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 5/27/2014. 2. Nomura, N., Sasamoto, S., Ishii, S., Date, T., Matsui, M., Ishizaki, R. Isolation of human cDNA clones of SKI and the SKI-related gene, SNO. Nucleic Acids Res. 17: 5489-5500, 1989. 3. Pearson-White, S., Crittenden, R. Proto-oncogene Sno expression, alternative isoforms and immediate early serum response. Nucleic Acids Res. 25: 2930-2937, 1997. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- SKIL (SKI-Like Oncogene (SKIL))
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别名
- SKIL
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背景
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Synonyms: E3 SUMO-protein ligase PIAS2, Androgen receptor-interacting protein 3, ARIP3, DAB2-interacting protein, DIP, E3 SUMO-protein transferase PIAS2, Msx-interacting zinc finger protein, Miz1, PIAS-NY protein, Protein inhibitor of activated STAT x, Protein inhibitor of activated STAT2, PIAS2, PIASX
Tissue Specificity: Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.
Background: Ski-like protein is a protein that in humans is encoded by the SKIL gene. The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene.
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分子量
- 77 kDa
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基因ID
- 6498
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UniProt
- P12757
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途径
- Positive Regulation of Response to DNA Damage Stimulus
抗原
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