APEX2 抗体 (AA 1-500)
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- 抗原 See all APEX2 抗体
- APEX2 (APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2))
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抗原表位
- AA 1-500
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This APEX2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS)
- 原理
- Anti-APEX2 Antibody Picoband®
- 交叉反应 （详细）
- No cross-reactivity with other proteins.
- 产品特性
- Anti-APEX2 Antibody Picoband® (ABIN7599449). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- E.coli-derived human APEX2 recombinant protein (Position: M1-G500).
- 亚型
- IgG
- Top Product
- Discover our top product APEX2 Primary Antibody
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anti-APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2) (AA 143-171) antibody
APEX2 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB23043 unconjugated
anti-APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2) (AA 1-518) antibodyAPEX2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2) (AA 301-400) antibodyAPEX2 适用: 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Burkovics, P., Szukacsov, V., Unk, I., Haracska, L.Human Ape2 protein has 3-5-prime exonuclease activity that acts preferentially on mismatched base pairs.Nucleic Acids Res. 34: 2508-2515, 2006. 2. Hadi, M. Z., Ginalski, K., Nguyen, L. H., Wilson, D. M., III.Determinants in nuclease specificity of Ape1 and Ape2, human homologues of Escherichia coli exonuclease III.J. Molec. Biol. 316: 853-866, 2002. 3. Tsuchimoto, D., Sakai, Y., Sakumi, K., Nishioka, K., Sasaki, M., Fujiwara, T., Nakabeppu, Y.Human APE2 protein is mostly localized in the nuclei and to some extent in the mitochondria, while nuclear APE2 is partly associated with proliferating cell nuclear antigen.Nucleic Acids Res. 29: 2349-2360, 2001. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- 抗原
- APEX2 (APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2))
- 别名
- APEX2 (APEX2 产品)
- 背景
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Synonyms: DNA- (apurinic or apyrimidinic site) lyase 2,3.1.-.-,4.2.99.18,AP endonuclease XTH2,APEX nuclease 2,APEX nuclease-like 2,Apurinic-apyrimidinic endonuclease 2,AP endonuclease 2,APEX2,APE2, APEXL2, XTH2,
Tissue Specificity: Highly expressed in brain and kidney. Weakly expressed in the fetal brain. .
Background: APEX2, also called apurinic/apyrimidinic endonuclease like-2, is a member of the apurinic/apyrimidinic (AP) family of endonucleases that initiate the repair of AP sites formed by spontaneous hydrolysis of the N-glycosylic bond, mutagen-induced base release, or damaged-base excision by a DNA repair glycosylase. RT-PCR detected APEX2 expression in HeLa cells, Jurkat cells, and human kidney, brain and fetal brain tissue. The APEX2 gene is mapped to chromosome Xp11.21. APEX2 participates in both nuclear and mitochondrial base excision repair (BER) and it can play a role in processing 3-prime-damaged termini or 3-prime-mismatched nucleotides. Additionally, APEX2 displayed weaker AP site-specific and 3-prime nuclease activities compared to APEX1.
- 分子量
- 57 kDa
- 基因ID
- 27301
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