CRBN 抗体 (AA 1-378)
Quick Overview for CRBN 抗体 (AA 1-378) (ABIN7599329)
抗原
See all CRBN 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-378
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原理
- Anti-CRBN Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-CRBN Antibody Picoband® (ABIN7599329). Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human CRBN recombinant protein (Position: M1-H378). Human CRBN shares 95% amino acid (aa) sequence identity with both mouse and rat CRBN.
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亚型
- IgG
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anti-Cereblon (CRBN) (AA 1-280) antibody
CRBN 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cereblon (CRBN) (AA 1-442) antibodyCRBN 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Cereblon (CRBN) (AA 35-84) antibodyCRBN 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 兔, Hamster, 猴, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL
1. Fischer, E. S., Bohm, K., Lydeard, J. R., Yang, H., Stadler, M. B., Cavadini, S., Nagel, J., Serluca, F., Acker, V., Lingaraju, G. M., Tichkule, R. B., Schebesta, M., and 9 others. Structure of the DDB1-CRBN E3 ubiquitin ligase in complex with thalidomide. Nature 512: 49-53, 2014. 2. Higgins, J. J., Hao, J., Kosofsky, B. E., Rajadhyaksha, A. M. Dysregulation of large-conductance Ca(2+)-activated K(+) channel expression in nonsyndromal mental retardation due to a cereblon p.R419X mutation. Neurogenetics 9: 219-223, 2008. 3. Higgins, J. J., Pucilowska, J., Lombardi, R. Q., Rooney, J. P. A mutation in a novel ATP-dependent Lon protease gene in a kindred with mild mental retardation. Neurology 63: 1927-1931, 2004. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- CRBN (Cereblon (CRBN))
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别名
- CRBN
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背景
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Synonyms: CRBN, AD-006, Protein cereblon
Background: Cereblon is a protein that in humans is encoded by the CRBN gene. This gene encodes a protein related to the Lon protease protein family. In rodents and other mammals this gene product is found in the cytoplasm localized with a calcium channel membrane protein, and is thought to play a role in brain development. Mutations in this gene are associated with autosomal recessive nonsyndromic cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene.
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分子量
- 51 kDa
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基因ID
- 51185
抗原
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