TOLLIP 抗体 (AA 1-274)
Quick Overview for TOLLIP 抗体 (AA 1-274) (ABIN7599202)
抗原
See all TOLLIP 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-274
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原理
- Anti-TOLLIP Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-TOLLIP Antibody Picoband® (ABIN7599202). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human TOLLIP recombinant protein (Position: M1-P274).
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亚型
- IgG
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anti-Toll Interacting Protein (TOLLIP) (AA 1-274) antibody
TOLLIP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 1-274) antibodyTOLLIP 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 1A5-2A3 unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 19-254) antibodyTOLLIP 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 1-274) antibodyTOLLIP 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 1-274) antibodyTOLLIP 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 221-250), (C-Term) antibodyTOLLIP 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB4409 unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 224-243), (C-Term) antibodyTOLLIP 适用: 人 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 28-261) antibodyTOLLIP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 86-163) antibodyTOLLIP 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 61-274) antibodyTOLLIP 适用: 人 WB, ELISA, IF, ICC 宿主: 小鼠 Monoclonal k1B17 unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bulut, Y., Faure, E., Thomas, L., Equils, O., Arditi, M. Cooperation of Toll-like receptor 2 and 6 for cellular activation by soluble tuberculosis factor and Borrelia burgdorferi outer surface protein A lipoprotein: role of Toll-interacting protein and IL-1 receptor signaling molecules in Toll-like receptor 2 signaling. J. Immun. 167: 987-994, 2001. 2. Burns, K., Clatworthy, J., Martin, L., Martinon, F., Plumpton, C., Maschera, B., Lewis, A., Ray, K., Tschopp, J., Volpe, F. Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor. Nature Cell Biol. 2: 346-351, 2000. 3. Lo, Y.-L. S., Beckhouse, A. G., Boulus, S. L., Wells, C. A. Diversification of TOLLIP isoforms in mouse and man. Mammalian Genome 20: 305-314, 2009. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- TOLLIP (Toll Interacting Protein (TOLLIP))
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别名
- TOLLIP
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背景
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Synonyms: Short transient receptor potential channel 4, TrpC4, Trp-related protein 4, hTrp-4, hTrp4, TRPC4
Tissue Specificity: Strongly expressed in placenta. Expressed at lower levels in heart, pancreas, kidney and brain. Expressed in endothelial cells. Isoform alpha was found to be the predominant isoform. Isoform beta was not found in pancreas and brain.
Background: TOLLIP(TOLL-Interacting Protein), is an inhibitory adaptor protein that in humans is encoded by the TOLLIP gene. Lo et al.(2009) stated that the TOLLIP gene maps to chromosome 11. The mouse gene maps to chromosome 7. By Western blot analysis of embryonic kidney cells, Burns et al.(2000) confirmed the binding of TOLLIP to IL1RAP, to a complex of IL1RAP-IL1R1, and to IL18R. Burns et al.(2000) proposed that IL1B stimulation induces aggregation of IL1Rs, recruitment of MYD88 followed by TOLLIP-IRAK complexes, and the phosphorylation of IRAK by MYD88. This leads to the dissociation of TOLLIP from IRAK, which can then transmit the IL1-induced signals.
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分子量
- 30 kDa
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基因ID
- 54472
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UniProt
- Q9H0E2
抗原
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