ANAPC5 抗体 (AA 1-266)
Quick Overview for ANAPC5 抗体 (AA 1-266) (ABIN7599186)
抗原
See all ANAPC5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-266
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原理
- Anti-Apc5/ANAPC5 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Apc5/ANAPC5 Antibody Picoband® (ABIN7599186). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Apc5/ANAPC5 recombinant protein (Position: M1-D266).
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亚型
- IgG
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anti-Anaphase Promoting Complex Subunit 5 (ANAPC5) (AA 1-755) antibody
ANAPC5 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Anaphase Promoting Complex Subunit 5 (ANAPC5) (AA 1-250) antibodyANAPC5 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Anaphase Promoting Complex Subunit 5 (ANAPC5) (AA 1-250) antibodyANAPC5 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Anaphase Promoting Complex Subunit 5 (ANAPC5) (Internal Region) antibodyANAPC5 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Anaphase Promoting Complex Subunit 5 (ANAPC5) (AA 2-232) antibodyANAPC5 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Anaphase Promoting Complex Subunit 5 (ANAPC5) (AA 1-755) antibodyANAPC5 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Anaphase Promoting Complex Subunit 5 (ANAPC5) (AA 156-185) antibodyANAPC5 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB24572 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Jorgensen, P. M., Graslund, S., Betz, R., Stahl, S., Larsson, C., Hoog, C. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene 262: 51-59, 2001. 2. Turnell, A. S., Stewart, G. S., Grand, R. J. A., Rookes, S. M., Martin, A., Yamano, H., Elledge, S. J., Gallimore, P. H. The APC/C and CBP/p300 cooperate to regulate transcription and cell-cycle progression. Nature 438: 690-695, 2005. 3. Yu, H., Peters, J.-M., King, R. W., Page, A. M., Hieter, P., Kirschner, M. W. Identification of a cullin homology region in a subunit of the anaphase-promoting complex. Science 279: 1219-1222, 1998. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ANAPC5 (Anaphase Promoting Complex Subunit 5 (ANAPC5))
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别名
- ANAPC5
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背景
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Synonyms: Neuropeptides B/W receptor type 1, G-protein coupled receptor 7, NPBWR1, GPR7
Tissue Specificity: Found in cerebellum and frontal cortex. Detected at high levels in hippocampus, amygdala and trachea, at moderate levels in fetal brain, pituitary gland and prostate. Not in caudate, accumbens, kidney or liver. Also detected at high levels in lung carcinoma.
Background: Anaphase-promoting complex subunit 5 is an enzyme that in humans is encoded by the ANAPC5 gene. This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.
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分子量
- 85 kDa
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基因ID
- 51433
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途径
- M Phase
抗原
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