Nanog 抗体 (AA 1-191)
Quick Overview for Nanog 抗体 (AA 1-191) (ABIN7599059)
抗原
See all Nanog (NANOG) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-191
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原理
- Anti-Nanog Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Nanog Antibody Picoband® (ABIN7599059). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived mouse Nanog recombinant protein (Position: M1-A191).
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亚型
- IgG
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anti-Nanog Homeobox (NANOG) antibody
NANOG 适用: 人 WB, ELISA, IF, IHC, ICC, IHC (p), IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 101-200) antibodyNANOG 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 1-305) antibodyNANOG 适用: 人 WB, ELISA, IF, FACS, ICC 宿主: 小鼠 Monoclonal 8A1D11 unconjugated
anti-Nanog Homeobox (NANOG) (AA 71-170) antibodyNANOG 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 15-49) antibodyNANOG 适用: 人 WB, ELISA, IF, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (Internal Region) antibodyVerified NANOG 适用: 人 WB, ELISA, IF, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 98-195) antibodyNANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A12 unconjugated
anti-Nanog Homeobox (NANOG) (AA 98-195) antibodyNANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Nanog Homeobox (NANOG) (AA 1-100) antibodyNANOG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Nanog Homeobox (NANOG) (AA 20-166) antibodyNANOG 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 1E6C4 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Chambers, I., Colby, D., Robertson, M., Nichols, J., Lee, S., Tweedie, S., Smith, A. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 113: 643-655, 2003. 2. Clark, A. T., Rodriguez, R. T., Bodnar, M. S., Abeyta, M. J., Cedars, M. I., Turek, P. J., Firpo, M. T., Pera, R. A. R. Human STELLAR, NANOG, and GDF3 genes are expressed in pluripotent cells and map to chromosome 12p13, a hotspot for teratocarcinoma. Stem Cells 22: 169-179, 2004. 3. Hart, A. H., Hartley, L., Ibrahim, M., Robb, L. Identification, cloning and expression analysis of the pluripotency promotingNanog genes in mouse and human. Dev. Dyn. 230: 187-198, 2004. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- Nanog (NANOG) (Nanog Homeobox (NANOG))
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别名
- Nanog
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背景
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Synonyms: Homeobox protein NANOG,Homeobox transcription factor Nanog,hNanog,NANOG,
Tissue Specificity: Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. .
Background: NANOG (pron. nanOg) is a transcription factor critically involved with self-renewal of undifferentiated embryonic stem cells. In humans, this protein is encoded by the NANOG gene. It is mapped to 12p13.31. NANOG is thought to be a key factor in maintaining pluripotency. Moreover, NANOG is also thought to function in concert with other factors such as POU5F1 (Oct-4) and SOX2 to establish ESC identity. The NANOG protein has been found to be a transcriptional activator for the Rex1 promoter, playing a key role in sustaining Rex1 expression. Knockdown of NANOG in embryonic stem cells results in a reduction of Rex1 expression, while forced expression of NANOG stimulates Rex1 expression.
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分子量
- 37 kDa
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基因ID
- 71950
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UniProt
- Q80Z64
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途径
- Stem Cell Maintenance
抗原
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