DDIT3 抗体 (AA 1-169)
Quick Overview for DDIT3 抗体 (AA 1-169) (ABIN7599026)
抗原
See all DDIT3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-169
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原理
- Anti-DDIT3 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-DDIT3 Antibody Picoband® (ABIN7599026). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human DDIT3 recombinant protein (Position: M1-A169).
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亚型
- IgG
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anti-DNA-Damage-Inducible Transcript 3 (DDIT3) (C-Term) antibody
DDIT3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 3 (DDIT3) (AA 1-169) antibodyDDIT3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 3 (DDIT3) (N-Term) antibodyDDIT3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 3 (DDIT3) (pSer30) antibodyDDIT3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 3 (DDIT3) (N-Term) antibodyDDIT3 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 3 (DDIT3) (AA 120-149), (C-Term) antibodyDDIT3 适用: 人, 小鼠 WB, IF, FACS 宿主: 兔 Polyclonal RB13788 unconjugated
anti-DNA-Damage-Inducible Transcript 3 (DDIT3) (AA 1-169) antibodyDDIT3 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 3 (DDIT3) (Ser301) antibodyDDIT3 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA-Damage-Inducible Transcript 3 (DDIT3) (AA 1-90) antibodyDDIT3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G3 unconjugated
anti-DNA-Damage-Inducible Transcript 3 (DDIT3) (AA 75-168) antibodyDDIT3 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aman, P., Ron, D., Mandahl, N., Fioretos, T., Heim, S., Arheden, K., Willen, H., Rydholm, A., Mitelman, F. Rearrangement of the transcription factor gene CHOP in myxoid liposarcomas with t(12,16)(q13,p11). Genes Chromosomes Cancer 5: 278-285, 1992. 2. Crozat, A., Aman, P., Mandahl, N., Ron, D. Fusion of CHOP to a novel RNA-binding protein in human myxoid liposarcoma. Nature 363: 640-644, 1993. 3. Endo, M., Mori, M., Akira, S., Gotoh, T. C/EBP homologous protein (CHOP) is crucial for the induction of caspase-11 and the pathogenesis of lipopolysaccharide-induced inflammation. J. Immun. 176: 6245-6253, 2006. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))
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别名
- DDIT3
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背景
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Synonyms: DNA damage-inducible transcript 3 protein, DDIT-3, C/EBP zeta, C/EBP-homologous protein, CHOP, C/EBP-homologous protein 10, CHOP-10, CCAAT/enhancer-binding protein homologous protein, Growth arrest and DNA damage-inducible protein GADD153, DDIT3, CHOP, CHOP10, GADD153
Tissue Specificity: Abundantly expressed in heart, pancreas, prostate, kidney, small intestine and peripheral blood leukocytes. Less abundant in placenta, lung, spleen, colon, thymus, testis and ovaries.
Background: DNA damage-inducible transcript 3, also known as C/EBP homologous protein (CHOP), is a pro-apoptotictranscription factor that is encoded by the DDIT3 gene. It is mapped to 12q13.3. This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
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分子量
- 29 kDa
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基因ID
- 1649
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UniProt
- P35638
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途径
- Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis
抗原
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