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Enolase 抗体

适用: 人 WB 宿主: 小鸡 Polyclonal unconjugated
产品编号 ABIN7595318
发货至: 中国
  • 抗原 See all Enolase 抗体
    Enolase
    适用
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    宿主
    • 3
    • 2
    • 2
    小鸡
    克隆类型
    • 4
    • 3
    多克隆
    标记
    • 6
    • 1
    This Enolase antibody is un-conjugated
    应用范围
    • 6
    • 5
    • 3
    • 2
    Western Blotting (WB)
    原理
    Chicken antibody to Enolase
    特异性
    Specific for Enolase (alpha beta gamma).
    交叉反应
    人, Marmoset, 小鼠, 大鼠
    交叉反应 (详细)
    Other species not yet tested.
    纯化方法
    Purified IgY
    免疫原
    A synthetic peptide from AA of 10-50 of human Enolase conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
    亚型
    IgY
    Top Product
    Discover our top product Enolase Primary Antibody
  • 应用备注
    WB. A dilution of 1 : 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
    储存条件
    4 °C,-20 °C
    储存方法
    Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8C for a shorter term.
    有效期
    12 months
  • 抗原
    Enolase
    Abstract
    Enolase 产品
    背景
    Function: Multifunctional enzyme that as well as its role in glycolysis plays a part in various processes such as growth control hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. Mammalian enolase is composed of 3 isozyme subunits alpha beta and gamma which can form homodimers or heterodimers which are cell-type and development-specific. ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding. In vitro interacts with several glycolytic enzymes including PKM2 PGM CKM and aldolase. Also binds troponin in vitro. Subcellular location: Cytoplasm. Cell membrane. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M-band. Tissue specificity: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. In striated muscle expression of ENO1 appears to be independent of fiber type.
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