Enolase 抗体
This anti-Enolase antibody is a 小鸡 多克隆 antibody detecting Enolase in WB. Suitable for 人.
Quick Overview for Enolase 抗体 (ABIN7595318)
抗原
See all Enolase 抗体
Enolase
适用
人
宿主
小鸡
克隆类型
多克隆
标记
This Enolase antibody is un-conjugated
应用范围
Western Blotting (WB)
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原理
- Chicken antibody to Enolase
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特异性
- Specific for Enolase (alpha beta gamma).
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交叉反应
- 人, Marmoset, 小鼠, 大鼠
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交叉反应 （详细）
- Other species not yet tested.
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纯化方法
- Purified IgY
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免疫原
- A synthetic peptide from AA of 10-50 of human Enolase conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
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亚型
- IgY
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anti-Enolase (C-Term) antibody
适用: 人, 小鼠, 大鼠 ELISA, WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enolase antibody适用: 人 ELISA, WB, IHC 宿主: 小鼠 Monoclonal NSE-P1 unconjugated
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应用备注
- WB. A dilution of 1 : 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
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储存条件
- 4 °C,-20 °C
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储存方法
- Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8C for a shorter term.
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有效期
- 12 months
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- Enolase
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背景
- Function: Multifunctional enzyme that as well as its role in glycolysis plays a part in various processes such as growth control hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. Mammalian enolase is composed of 3 isozyme subunits alpha beta and gamma which can form homodimers or heterodimers which are cell-type and development-specific. ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding. In vitro interacts with several glycolytic enzymes including PKM2 PGM CKM and aldolase. Also binds troponin in vitro. Subcellular location: Cytoplasm. Cell membrane. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M-band. Tissue specificity: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. In striated muscle expression of ENO1 appears to be independent of fiber type.
抗原
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