Enolase 抗体
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北京 101111
Quick Overview for Enolase 抗体 (ABIN459288)
抗原
See all Enolase 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Sheep anti Rabbit Enolase
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特异性
- Purified hyperimmune Sheep IgG lyophilised from a solution in phosphate buffered saline.
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交叉反应 （详细）
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
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产品特性
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer's own choice.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Enolase isolated and purified from rabbit muscle. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Enolase (AA 340-420) antibody
适用: 人, 小鼠, 大鼠 ELISA, WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enolase (C-Term) antibody适用: 人, 小鼠, 大鼠 ELISA, WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enolase antibody适用: 人, 小鼠, 小鸡 ELISA, WB, IHC 宿主: 小鼠 Monoclonal NSE-P1 unconjugated
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应用备注
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Purified hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline . No preservative added, as it may interfere with the antibody activity. No foreign protein added.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Enolase
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UniProt
- G1U6X0
抗原
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