HCAR2 抗体 (Extracellular, Loop 1) (FITC)
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Quick Overview for HCAR2 抗体 (Extracellular, Loop 1) (FITC) (ABIN7582038)
抗原
See all HCAR2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 82-94, Extracellular, Loop 1
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原理
- A Rabbit Polyclonal Antibody to GPR109A/HCAR2 (extracellular) conjugated to the fluorescent dye FITC.
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序列
- (C)DNYVHNWDWR FGG
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预测反应
- Rat - 11 out of 13 amino acid residues identical, Human - 9 out of 13 amino acid residues identical Note: it is not expected to recognize the highly homologous human protein HCAR3 (Accession P49019) HCAR3 has no mouse,rat orthologs
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产品特性
- Anti-GPR109A/HCAR2 (extracellular) Antibody (ABIN7237697, ABIN7316621 and ABIN7316622) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize GPR109A from rat, mouse and human samples. Anti-GPR109A/HCAR2 (extracellular)-FITC Antibody (ABIN7237697, ABIN7316621 and ABIN7316622-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)DNYVHNWDWRFGG, corresponding to amino acid residues 82 - 94 of mouse HCAR2
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亚型
- IgG
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anti-Hydroxycarboxylic Acid Receptor 2 (HCAR2) (AA 82-94), (Extracellular), (Loop 1) antibody
HCAR2 适用: 小鼠 WB, IHC, FACS, LCI 宿主: 兔 Polyclonal unconjugated
anti-Hydroxycarboxylic Acid Receptor 2 (HCAR2) antibodyHCAR2 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hydroxycarboxylic Acid Receptor 2 (HCAR2) (AA 250-350) antibodyHCAR2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Hydroxycarboxylic Acid Receptor 2 (HCAR2) (AA 200-228) antibodyHCAR2 适用: 人 WB 宿主: 兔 Polyclonal RB31358 unconjugated
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应用备注
- The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7235778)
Blocking Peptide: (ABIN7235778)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 15 μL or 50 μL double distilled water (DDW), depending on the sample size.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- HCAR2 (Hydroxycarboxylic Acid Receptor 2 (HCAR2))
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别名
- HCAR2
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背景
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Synonyms: Hydroxycarboxylic Acid Receptor 2, Niacin Receptor 1, G-Protein Coupled Receptor HM74A, HM74A, Nicotinic acid receptor, PUMAG
Description: Hydroxy-carboxylic acid receptors (HCA1-3) are a family of G-protein-coupled receptors that regulate lipolysis. HCAR2 (GPR109A) was identified as the receptor for nicotinic acid (niacin, vitamin B3) and beta-hyroxybutyrate (β-OHB), a ketone body synthesized by the liver under negative energy balance1.HCAR2 is mainly expressed on adipocytes and immune cells and in lower levels in retinal pigmented and colonic epithelial cells, microglia and normal mammary tissue. High levels of HCAR2 can be found in immune cells such as macrophages in response to the presence of interferon-γ, TNF-α and hypoxia2.HCAR2 activity in adipocytes promotes inhibition of adenylate cyclase and decrease cAMP levels. HCAR2 activity leads to a rapid decrease in lipolysis and reduction in the release of fatty acids from the adipocytes3.Activation of HCAR2 has also been associated with anti-inflammatory effects in different disease states such as sepsis, diabetes and obesity. Interaction of HCAR2 with niacin and β-OHB under this conditions lead to variety of signaling events involving inflammatory downstream targets4.
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基因ID
- 80885
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UniProt
- Q9EP66
抗原
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