GPR4 抗体 (Extracellular) (FITC)
Quick Overview for GPR4 抗体 (Extracellular) (FITC) (ABIN7582035)
抗原
See all GPR4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 162-177, Extracellular
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原理
- A Rabbit Polyclonal Antibody to GPR4 (extracellular) conjugated to the fluorescent dye FITC
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序列
- (C)RDRYNHTF(S)FEKFPME
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特异性
- 2nd extracellular loop
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预测反应
- Rat,mouse,human - 15,16 amino acid residues identical
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产品特性
- Anti-GPR4 (extracellular) Antibody (ABIN7043160, ABIN7044423 and ABIN7044424) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize GPR4 from mouse, rat and human samples. Anti-GPR4 (extracellular)-FITC Antibody (ABIN7043160, ABIN7044423 and ABIN7044424-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize GPR4 from mouse, rat and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)RDRYNHTF(S)FEKFPME, corresponding to amino acid residues 162-177 of rat GPR4
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亚型
- IgG
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anti-G Protein-Coupled Receptor 4 (GPR4) (Internal Region) antibody
GPR4 适用: 人, 大鼠, 小鼠 ELISA, WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (C-Term) antibodyGPR4 适用: 人, 犬, 猴 IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (AA 285-362) antibodyGPR4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (Extracellular Domain) antibodyGPR4 适用: 人, 大鼠, 小鼠, 犬, Pig, Cow, 猴, Bat, 兔 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (Cytoplasmic Domain) antibodyGPR4 适用: 人, 犬, Pig, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (AA 162-177), (Extracellular) antibodyGPR4 适用: 大鼠 WB, IHC, FACS, LCI 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (AA 201-250) antibodyGPR4 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (Internal Region) antibodyGPR4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (AA 196-224) antibodyGPR4 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB43856 unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: N/A
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说明
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Negative Control: (ABIN7582044)
Blocking Peptide: (ABIN7235415)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 15 μL or 50 μL double distilled water (DDW), depending on the sample size.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GPR4 (G Protein-Coupled Receptor 4 (GPR4))
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别名
- GPR4
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背景
- Acid-sensing G-protein coupled receptor 4, GPR19,G-protein coupled receptor 4 (GPR4) belongs to a protein family comprised of 3 related G-protein coupled receptors (GPCRs): GPR4, OGR1/GPR68 and TDAG8/GPR65. Like all GPCRs, GPR4's structure is composed of seven-transmembrane-spanning domains linked by an N-terminal extracellular domain and a C-terminal intracellular domain2.GPR4 responds to pH changes and is activated by a physiological increase in extracellular H+ through several signaling pathways including- the G12/13-protein/Rho, the Gq/PLC, and the Gs-protein/cAMP pathway1,2. It is fully activated at pH levels of 6.8 and lower and is inactive at pH levels of 7.5 and higher3,4.Recent studies show that the pH -sensing GPCRs also have the ability to regulate cancer cell metastasis and proliferation, immune cell function, inflammation, blood vessel formation, endothelial barrier function thus making them an important therapeutic target for different diseases. Overexpression of GPR4 in human cancer cells shows involvement in driving or maintaining tumor formation.GPR4 is expressed in several tissues including lung, vascular endothelial cells, kidney, heart, liver, skeletal muscle, ovary, and placenta.
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基因ID
- 308408
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UniProt
- Q4KLH9
抗原
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