GPR4 抗体 (2nd Extracellular Loop)
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Quick Overview for GPR4 抗体 (2nd Extracellular Loop) (ABIN7043160)
抗原
See all GPR4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- 2nd Extracellular Loop, AA 162-177
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原理
- A Rabbit Polyclonal Antibody to GPR4
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat,mouse,human - 15,16 amino acid residues identical
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产品特性
- Anti-GPR4 (extracellular) Antibody (ABIN7043160, ABIN7044423 and ABIN7044424) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize GPR4 from mouse, rat, and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)RDRYNHTF(S)FEKFPME, corresponding to amino acid residues 162-177 of rat GPR4
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亚型
- IgG
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anti-G Protein-Coupled Receptor 4 (GPR4) (Internal Region) antibody
GPR4 适用: 人, 大鼠, 小鼠 ELISA, WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (C-Term) antibodyGPR4 适用: 人, 犬, 猴 IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (AA 285-362) antibodyGPR4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (Extracellular Domain) antibodyGPR4 适用: 人, 大鼠, 小鼠, 犬, Pig, Cow, 猴, Bat, 兔 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (Cytoplasmic Domain) antibodyGPR4 适用: 人, 犬, Pig, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (AA 196-224) antibodyGPR4 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB43856 unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (AA 201-250) antibodyGPR4 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (Internal Region) antibodyGPR4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (2nd Extracellular Loop), (AA 162-177) antibody (FITC)GPR4 适用: 大鼠 FACS, LCI 宿主: 兔 Polyclonal FITC
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应用备注
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WB: 1:200
FC: 2.5 μg per reaction
ICC: The optimal concentration should be determined by the user
IHC: 1:400
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7235415)
Blocking Peptide: (ABIN7235415)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- GPR4 (G Protein-Coupled Receptor 4 (GPR4))
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别名
- GPR4
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背景
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Synonyms: Acid-sensing G-protein coupled receptor 4, GPR19
Description: G-protein coupled receptor 4 (GPR4) belongs to a protein family comprised of 3 related G-protein coupled receptors (GPCRs): GPR4, OGR1/GPR68 and TDAG8/GPR65. Like all GPCRs, GPR4's structure is composed of seven-transmembrane-spanning domains linked by an N-terminal extracellular domain and a C-terminal intracellular domain2.GPR4 responds to pH changes and is activated by a physiological increase in extracellular H+ through several signaling pathways including- the G12/13-protein/Rho, the Gq/PLC, and the Gs-protein/cAMP pathway1,2. It is fully activated at pH levels of 6.8 and lower and is inactive at pH levels of 7.5 and higher3,4.Recent studies show that the pH -sensing GPCRs also have the ability to regulate cancer cell metastasis and proliferation, immune cell function, inflammation, blood vessel formation, endothelial barrier function thus making them an important therapeutic target for different diseases. Overexpression of GPR4 in human cancer cells shows involvement in driving or maintaining tumor formation.GPR4 is expressed in several tissues including lung, vascular endothelial cells, kidney, heart, liver, skeletal muscle, ovary, and placenta.
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基因ID
- 308408
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NCBI登录号
- NM_005282
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UniProt
- Q4KLH9
抗原
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