ACE2 抗体 (Extracellular) (FITC)
Quick Overview for ACE2 抗体 (Extracellular) (FITC) (ABIN7582000)
抗原
See all ACE2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 197-213, Extracellular
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原理
- A Rabbit Polyclonal Antibody to ACE2 (extracellular) conjugated to the fluorescent dye FITC
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序列
- (C)EDYGDYWRGD YEVNGVD
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特异性
- Extracellular, N-term.
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预测反应
- Rat - 14 out of 17 amino acid residues identical,mouse - 13 out of 17 amino acid residues identical
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产品特性
- Anti-ACE2 (extracellular) Antibody (ABIN7237591, ABIN7316539 and ABIN7316540) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize ACE2 from mouse, rat and human samples. Anti-ACE2 (extracellular)-FITC Antibody (ABIN7237591, ABIN7316539 and ABIN7316540-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)EDYGDYWRGDYEVNGVD, corresponding to amino acid residues 197 - 213 of human ACE2
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亚型
- IgG
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anti-Angiotensin I Converting Enzyme 2 (ACE2) (AA 601-700) antibody
ACE2 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme 2 (ACE2) antibodyACE2 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal AC18F unconjugated
anti-Angiotensin I Converting Enzyme 2 (ACE2) (AA 18-250) antibodyACE2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme 2 (ACE2) (AA 93-464) antibodyACE2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme 2 (ACE2) (AA 392-739) antibodyACE2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme 2 (ACE2) (N-Term) antibodyVerified ACE2 适用: 人 WB, IHC, ELISA, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme 2 (ACE2) (AA 773-805), (C-Term) antibodyACE2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB4819 unconjugated
anti-Angiotensin I Converting Enzyme 2 (ACE2) (AA 140-172) antibodyACE2 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme 2 (ACE2) (AA 59-90) antibodyACE2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme 2 (ACE2) (AA 351-450) antibodyACE2 适用: 人, 小鼠 WB, ELISA, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: N/A
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说明
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Negative Control: (ABIN7582044)
Blocking Peptide: (ABIN7234723)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 50 μL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))
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别名
- ACE2
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背景
- Angiotensin-Converting Enzyme 2, ACE-Related Carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15,The renin-angiotensin system is a central mechanism for blood pressure regulation through a diverse system of hormones and receptors. Angiotensin-converting enzyme (ACE) is an ectoenzyme that plays a role in the generation of Ang II by catalyzing the extracellular conversion of the decapeptide Ang I. Moreover, ACE degrades active bradykinin (BK), which play an important role in the control of blood pressure. ACE2 is an ACE homolog that was discovered in 2000 when two independent research groups cloned homologous ACE that could convert Ang I to Ang (1-9). Sequence analysis suggests that ACE and ACE2 exhibit 42 % amino acid homology and ACE2 has evolved through gene duplication1.ACE2 consist of N-terminal peptidase domain with seven potential N-linked glycosylation sites that bears the characteristic HEXXH motif of M2 metalloproteases. It is mainly expressed in the small intestine, testis, kidneys, heart, thyroid and adipose tissue and in the lungs. ACE 2 has a protective role in the cardiovascular system and in alveolar epithelial cells. In the lungs ACE2 has numerous physiological functions, most of which are protective against lung injury2. ACE 2 was identified as the receptor for highly pathogenic SARS (severe acute respiratory syndrome) coronavirus including the SARS-CoV-2 that cause the coronavirus disease 2019 (COVID-19) global pandemic. SARS virus binding downregulates the cellular expression of ACE2, and the binding induces clathrin-dependent internalization of virus/receptor (SARS/ACE2) complex. Not only has ACE2 facilitated the invasion of SARS virus for rapid replication, but also ACE2 is depleted from the cell membrane and therefore the damaging effects of Ang II are enhanced, resulting in acute deterioration of lung tissues3.
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基因ID
- 59272
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UniProt
- Q9BYF1
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途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
抗原
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