Nerve Growth Factor 抗体
Quick Overview for Nerve Growth Factor 抗体 (ABIN7581918)
抗原
See all Nerve Growth Factor (NGF) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- A Mouse Monoclonal Antibody to NGF and proNGF Designed to Neutralize their Biological Functions
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序列
- Highly purified 2.5S mouse NGF
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交叉反应 （详细）
- The antibody has minimal cross-reactivity with BDNF, NT-3, and NT-4/5.
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预测反应
- NGF,proNGF from human,rat,mouse samples
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产品特性
- Highly specific monoclonal antibody directed against an epitope of mouse NGF. Mouse NGF/proNGF Neutralizing Antibody (ABIN7581918) can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF. It was designed to recognize NGF and proNGF from human, rat and mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- Highly purified 2.5S mouse NGF
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亚型
- IgG1, IgG1 kappa
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anti-Nerve Growth Factor (NGF) antibody
NGF 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) (AA 19-241) antibodyNGF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) (AA 162-173), (N-Term) antibodyNGF 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) antibodyNGF 适用: 人, 大鼠, 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) (AA 19-239) antibodyNGF 适用: 人 WB, ICC, IF 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Nerve Growth Factor (NGF) (AA 122-218) antibodyNGF 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) (AA 122-241) antibodyNGF 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) antibodyNGF 适用: 人 WB, IHC, ELISA 宿主: 兔 Monoclonal 5F10 unconjugated Recombinant Antibody
anti-Nerve Growth Factor (NGF) (N-Term) antibodyNGF 适用: 人, 大鼠, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) antibody (Biotin)NGF 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
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应用备注
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Antigen preadsorption control: NA
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: NA
Application Dilutions Western blot wb: NA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 50 μL or 0.25 mL double distilled water (DDW), depending on the sample size.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Nerve Growth Factor (NGF)
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别名
- NGF
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背景
- Nerve growth factor,The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and the PNS.1 NGF, the first member of the family to be discovered, was originally purified as a factor able to support survival of sympathetic and sensory spinal neurons in culture.2 It is synthesized and secreted by sympathetic and sensory target organs and provides trophic support to neurons as they reach their final target.3 Neurotrophin secretion also increases in the nervous system following injury. Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF constitutively, however direct trauma and induced cytokines combine to increase neurotrophin production in these cells after injury.4 NGF is purified in three forms: the 7S, 2.5S and β. The 7S, 130 kDa, form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two α, one β and two γ subunits. The name is derived from its sedimentation co-efficient, 7S. The biologically active subunit is the β, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino acids shorter than the β form, because of proteolysis that occurs during the purification process.6 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.7 There is a 95.8 % homology between the rat and mouse forms, and a 85 % homology between the human and mouse.NGF has been shown to regulate neuronal survival, development function and plasticity.8 Recently, involvement of NGF in processes not involving neuronal cells has been shown, such as asthma,9 psoriasis10 and wound healing.11 The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75, which binds all the neurotrophins.12
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基因ID
- 18049
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UniProt
- P01139
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途径
- Regulation of Cell Size
抗原
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