电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

Nerve Growth Factor 抗体

NGF 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN465644
发货至: 中国
  • 抗原 See all Nerve Growth Factor (NGF) 抗体
    Nerve Growth Factor (NGF)
    适用
    • 72
    • 44
    • 43
    • 10
    • 8
    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    宿主
    • 76
    • 17
    • 4
    • 2
    • 1
    • 1
    克隆类型
    • 73
    • 28
    多克隆
    标记
    • 63
    • 14
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Nerve Growth Factor antibody is un-conjugated
    应用范围
    • 64
    • 42
    • 34
    • 19
    • 10
    • 8
    • 7
    • 7
    • 6
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Neutralization (Neut)
    特异性
    Human beta-NGF
    纯化方法
    Immunoaffinity purified
    免疫原
    E.coli derived recombinant Human beta-NGF.

    Type of Immunogen: Cell extract
  • 应用备注
    Approved: ELISA (0.5 - 2 μg/mL), Neut (5 - 8 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 0.5 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results were achieved without antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human beta-NGF (1 ng/mL), a concentration of 0.05-0.08 μg/mL of this antibody is required. ELISA: To detect Human beta-NGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human beta-NGF (LS-C104735) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human beta-NGF. Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Sterile water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.2
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    Nerve Growth Factor (NGF)
    别名
    NGF (NGF 产品)
    别名
    Beta-NGF antibody, HSAN5 antibody, NGFB antibody, Ngfb antibody, ngfb antibody, ns:zft386 antibody, xx:zft386 antibody, zgc:109730 antibody, beta-NGF antibody, hsan5 antibody, beta-ngf antibody, NGF antibody, ngf antibody, nerve growth factor antibody, nerve growth factor b (beta polypeptide) antibody, nerve growth factor S homeolog antibody, NGF antibody, Ngf antibody, ngfb antibody, ngf antibody, ngf.S antibody
    背景
    Name/Gene ID: NGF
    Family: NGF Beta

    Synonyms: NGF, Beta-nerve growth factor, ProNGF, Beta-NGF, HSAN5, NGFB
    基因ID
    4803
    UniProt
    P01138
    途径
    Regulation of Cell Size
You are here:
客服