CHRNB1 抗体 (Extracellular)
Quick Overview for CHRNB1 抗体 (Extracellular) (ABIN7581859)
抗原
See all CHRNB1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 68-80, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β1
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序列
- (C)EKDEEMSTKV YLD
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特异性
- Extracellular, N-terminus
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预测反应
- Mouse,human - identical
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产品特性
- Highly specific antibody directed against an epitope of rat nAChR β1. Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody (ABIN7581859) can be used in western blot analysis. As the antibody recognizes an extracellular epitope, it can be used to detect the receptor in living cells. It has been designed to nAChRβ1 from rat, mouse and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)EKDEEMSTKVYLD, corresponding to amino acid residues 68-80 of rat nAChRbeta1
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亚型
- IgG
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anti-Nicotinic Acetylcholine Receptor beta (CHRNB1) (AA 24-244) antibody
CHRNB1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nicotinic Acetylcholine Receptor beta (CHRNB1) (N-Term) antibodyCHRNB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nicotinic Acetylcholine Receptor beta (CHRNB1) (AA 274-501) antibodyCHRNB1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nicotinic Acetylcholine Receptor beta (CHRNB1) (AA 131-230) antibodyCHRNB1 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Nicotinic Acetylcholine Receptor beta (CHRNB1) (N-Term) antibodyCHRNB1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nicotinic Acetylcholine Receptor beta (CHRNB1) (Internal Region) antibodyVerified CHRNB1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Nicotinic Acetylcholine Receptor beta (CHRNB1) (AA 24-128) antibodyCHRNB1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4G4 unconjugated
anti-Nicotinic Acetylcholine Receptor beta (CHRNB1) (AA 25-235) antibodyCHRNB1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- CHRNB1 (Nicotinic Acetylcholine Receptor beta (CHRNB1))
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别名
- CHRNB1
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背景
- NAChR β1, ACHRB,Neuronal nicotinic acetylcholine receptors (nAChRs) belong to the superfamily of ligand-gated ion channels and are widely expressed throughout the central and peripheral nervous systems. nAChRs play crucial roles in modulating a wide range of higher cognitive functions by mediating presynaptic, postsynaptic, and extrasynaptic signaling1.nAChRs are formed by the assembly of five transmembrane subunits, selected from a pool of 17 homologous polypeptides (α1-10, β1-4, γ, δ, and ε). There are many nAChR subtypes, each consisting of a specific combination of subunits, which mediate diverse physiological functions. They are widely expressed in the central nervous system, while, in the periphery, they mediate synaptic transmission at the neuromuscular junction and ganglia. nAChRs are also found in non-neuronal/non-muscle cells (keratinocytes, epithelia, macrophages, etc.)2. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, three conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore3.While most β subunits are neuronal, the β1 subunit forms functional receptors along with other subunits in the muscle2. β1 subunit seems to be involved in myasthenia gravis (MG)4, an acquired autoimmune disease usually characterized by the presence of circulating autoantibodies that bind to and destroy muscle nAChRs5.
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基因ID
- 24261
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UniProt
- P25109
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途径
- Synaptic Membrane
抗原
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