CALHM2 抗体 (Extracellular)
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- 抗原 See all CALHM2 products
- CALHM2 (Calcium Homeostasis Modulator 2 (CALHM2))
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抗原表位
- AA 133-146, Extracellular
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CALHM2 antibody is un-conjugated
- 应用范围
- Western Blotting (WB), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (IHC)
- 原理
- A Rabbit Polyclonal antibody to CALHM2 (extracellular)
- 序列
- (C)SEFVDPSSLT AGDK
- 特异性
- Extracellular, 2nd loop.
- 预测反应
- Rat - 13 out of 14 amino acid residues identical Human - 11 out of 14 amino acid residues identical
- 产品特性
- Anti-CALHM2 (extracellular) Antibody (ABIN7581840) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CALHM2 from mouse, rat and human samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
- (C)SEFVDPSSLTAGDK, corresponding to amino acid residues 133 - 146 of mouse CALHM2
- 亚型
- IgG
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anti-Calcium Homeostasis Modulator 2 (CALHM2) (AA 1-323) antibody
CALHM2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Calcium Homeostasis Modulator 2 (CALHM2) antibodyCALHM2 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:200
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- 0.2 mL double distilled water (DDW).
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- CALHM2 (Calcium Homeostasis Modulator 2 (CALHM2))
- 别名
- CALHM2 (CALHM2 产品)
- 背景
- Calcium homeostasis modulator protein 2, Protein FAM26B, FAM26B,Calcium homeostasis modulator protein 2 (CALHM2) is a member of the CALHM channel family, a group of transmembrane proteins that play essential roles in calcium ion transport and homeostasis. The CALHM family consist in humans of six members: CALHM1 to CALHM6.1CALHM2 functions as a calcium-permeable ion channel. It allows calcium ions to pass through the cell membrane in response to specific stimuli, such as changes in membrane potential or extracellular calcium concentration.2,3CALHM2 is composed of 323 amino acids and assembles as an undecamer with each protomer containing a large N-terminal transmembrane (TM) domain with 4 TM helices, an intracellular C-terminal domain, and a small extracellular linker region. 2,3CALHMs permeate ions and ATP in a voltage-dependent manner to modulate neuronal excitability. CALHM2 has been shown to accommodate lipids in its hydrophobic channel in order to regulate channel function.2,3Ion and ATP permeation by CALHM proteins play a role in the physiology of cognition, depression, gustatory signaling, and the pathology of Alzheimer's disease.Specifically, CALHM2 expression was increased in Alzheimer's disease (AD) patients and in an AD model in mice. Knockout of CALHM2 improved Aβ plaque deposition, reduced neuroinflammation, and improved cognitive impairment in an AD mice model.4Moreover, microglial CALHM2 may have a central role in both chronic inflammatory diseases (like AD) and in acute inflammatory reactions and neuroinflammation.4
- 基因ID
- 72691
- UniProt
- Q8VEC4
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