C3AR1 抗体 (Extracellular)
Quick Overview for C3AR1 抗体 (Extracellular) (ABIN7581833)
抗原
See all C3AR1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 172-186, Extracellular
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原理
- A Rabbit Polyclonal antibody to C3aR1 (extracellular)
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序列
- CRYNFDSSRS YDYWD
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特异性
- Extracellular, 2nd loop.
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预测反应
- Rat - 13 out of 15 amino acid residues identical It will not recognize C3aR1 from human samples
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产品特性
- Anti-C3aR1 (extracellular) Antibody (ABIN7581833) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize C3aR1 from mouse and rat samples. The antibody will not recognize C3aR1 from human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- CRYNFDSSRSYDYWD, corresponding to amino acid residues 172 - 186 of mouse C3aR1
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亚型
- IgG
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anti-Complement Component 3a Receptor 1 (C3AR1) (AA 1-482) antibody
C3AR1 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-Complement Component 3a Receptor 1 (C3AR1) (AA 401-482) antibodyC3AR1 适用: 人, 小鼠 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 3a Receptor 1 (C3AR1) (C-Term) antibodyC3AR1 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 3a Receptor 1 (C3AR1) (AA 160-340) antibodyC3AR1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 3a Receptor 1 (C3AR1) (AA 1-482) antibodyC3AR1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 3a Receptor 1 (C3AR1) antibodyC3AR1 适用: 人 FACS, IHC (fro) 宿主: 小鼠 Monoclonal HC3aRZ8 unconjugated
anti-Complement Component 3a Receptor 1 (C3AR1) antibody (PE)C3AR1 适用: 人 FACS 宿主: 小鼠 Monoclonal HC3aRZ8 PE
anti-Complement Component 3a Receptor 1 (C3AR1) antibodyC3AR1 适用: 人 FACS, ELISA 宿主: 兔 Chimeric 3G7 unconjugated Recombinant Antibody
anti-Complement Component 3a Receptor 1 (C3AR1) antibodyC3AR1 适用: 大鼠 IHC, FACS, IHC (fro) 宿主: 小鼠 Monoclonal 74-rC3aRZ1 unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:200
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- C3AR1 (Complement Component 3a Receptor 1 (C3AR1))
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别名
- C3AR1
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背景
- Complement C3a Receptor 1, C3AR, C3a Anaphylatoxin Chemotactic Receptor,The innate immune system identifies pathogens by recognizing known elements. This response ultimately leads to the production of proinflammatory substances and the activation of phagocytic neutrophils and macrophages1. Activation of the complement system is an important initial even in the response against pathogens. This system includes some thirty different proteins that act together to form a complex1.Three different pathways are known to activate the complement. The C3 complement plays a key role in all three pathways leading to the activation of the complement. Produced in the liver, C3 is the most abundant complement protein in the serum. It is composed of two polypeptides, an α and β chain. Cleavage of C3 leads to the production of C3b and C3a1.C3a binds to C3a receptor, a G-protein coupled receptor which couples to Gi upon activation. Like all other members of the superfamily, C3a receptor has seven transmembrane domains with an extracellular N-terminus and intracellular C-terminal tail1. The receptor has two glycosylation sites, an unusually large extracellular loop with a sulfated tyrosine residue important for ligand recognition and binding2,3.C3a receptor is expressed in cells of myeloid origin like neutrophils, macrophages and dendritic cells to name a few2. It is also detected in astrocytes from inflamed brain4, endothelial cells5 and activated human T cells6. mRNA of C3a receptor is detected in lung, liver, kidney, brain heart, muscle and testis2,7.
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基因ID
- 12267
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UniProt
- O09047
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途径
- Complement System
抗原
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