Western Blotting (WB), ELISA, Immunofluorescence (IF)
原理
Rabbit anti-HA-tag Antibody, animal-free mAb
特异性
This is an antibody developed by antibody phage display technology using a human naive antibody gene library and recombinant HA-tag antigen. For this antibody both the heavy and light chains are cloned and expressed, generating full-length antibodies.
交叉反应 (详细)
No known cross reactivity.
产品特性
This antibody can be detected with anti-rabbit Fc secondary antibodies.
纯化方法
Protein A purification
质量等级
Animal-Free
免疫原
No immunization, animal-free antibody development. Antigen: synthetic HA peptide corresponding to HA-tag (Human influenza hemagglutinin), aa 98-106, YPYDVPDYA
Western Blot: 0.2-5 μg/mL ELISA: 1-12 μg/mL as coating antibody 0.5-5 μg/mL as detection antibody IF: 0.1 - 10 μg/mL Optimal working dilution should be determined by the investigator
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
PBS, pH 7.4, 0,02 % ProClin300
储存液
ProClin
注意事项
This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.