Red Blood Cell 抗体 (Magnetic Particles)
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Quick Overview for Red Blood Cell 抗体 (Magnetic Particles) (ABIN7566001)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Red Blood Cell Depletion Magnetic Beads
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产品特性
- Synonyms: Red Blood Cell Depletion Magnetic Beads, Anti-RBC antibody, Red blood cell antibody, Antibody for hemagglutination, rabbit anti RBC, rabbit antibody to human red blood cells (RBC), haemolysin, hemolysin, erythrocytes sensitizing agent, anti-erythrotyes, anti-erythrocytes antibody, magnetic bead, nanoparticles, paramagnetic, Magnetic bead-conjugated
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免疫原
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Immunogen: Human washed pooled Red Blood Cells (RBC)
Immunogen Type: Other
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应用备注
- Application Note: Red Blood Cell Depletion Magnetic Beads have been tested in binding assays for separation of Red Blood Cells, from whole blood. Remaining white blood cells can be used for downstream assays such as immunoassays, Western blots, FACS, and immunohistochemistry. For 100 μL of whole, fresh blood cells add 75 μL of Depletion beads, expecting 75 - 95 % depletion of RBC after one pass. Optimal amount for applications should be determined by the researcher. Flow Cytometry Dilution: User Optimized Western Blot Dilution: User Optimized Other: User Optimized
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限制
- 仅限研究用
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浓度
- 20 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to opening. DO NOT FREEZE. The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
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有效期
- 12 months
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- Red Blood Cell (RBC)
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别名
- RBC
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背景
- Background: Red Blood Cell Depletion Magnetic Beads recognizes human red blood cells. Red Blood Cell Depletion Magnetic Beads is recommended for the depletion of human Red Blood Cells in samples prior to downstream immunological and non-immunological assays and has been tested in Flow Cytometry. Some additional assays could include Colony Forming Unit (CFU) Assay and Immunohistochemistry among others. These magnetic beads are uniform, non-aggregating, super-paramagnetic beads consisting of a ferric oxide core functionalized with various silane groups. The beads have a large surface area with high capture efficiencies. Bead mean diameter is ~0.5 μm, bead concentration is 20 mg/mL.
抗原
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