(1 reference)-
- 抗原 See all Red Blood Cell (RBC) products
- Red Blood Cell (RBC)
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适用
- 绵羊
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Red Blood Cell antibody is un-conjugated
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应用范围
- Cellular Assay (CA)
- 产品特性
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Blood Product Sterility: Non-sterile
Concentration Definition: by UV absorbance at 280 nm Blood Product Type: Red Blood Cells - 纯化方法
- This product is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
- Sheep washed pooled Red Blood Cells (RBC)
- 亚型
- IgG
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anti-Red Blood Cell (RBC) antibody
RBC 适用: 人 H, CyTox, IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Suitable for agglutination of cells on titer plates. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Restore with deionized water (or equivalent)
- 浓度
- 10.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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: "Mitochondrial Fission Promotes the Continued Clearance of Apoptotic Cells by Macrophages." in: Cell, Vol. 171, Issue 2, pp. 331-345.e22, (2017) (PubMed).
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: "Mitochondrial Fission Promotes the Continued Clearance of Apoptotic Cells by Macrophages." in: Cell, Vol. 171, Issue 2, pp. 331-345.e22, (2017) (PubMed).
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- 抗原
- Red Blood Cell (RBC)
- 别名
- Red Blood Cell RBC (RBC 产品)
- 背景
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Anti-SHEEP Red Blood Cell Antibody may be used in hemagglutination assays. Anti-SHEEP Red Blood Cell Antibody is used to sensitize erythrocytes and quantitate agglutination. Haemagglutination assay or HA is a method of quantification for viruses or bacteria by hemagglutination. Some viral families and many bacteria have envelope or surface proteins which are able to agglutinate (stick to) human or animal red blood cells (RBC) and bind to N-acetylneuraminic acid. As each of the agglutinating molecule attaches to multiple RBCs, a lattice-structure will form. Normally, a virus dilution (e.g. 2-fold from 1:4 to 1:4096) will be applied to an RBC dilution (e.g. 0.1% to 0.7% in steps of 0.2%) for approx. 30 min, often at 4° C, otherwise viruses with neuraminidase activity will detach the virus from the RBCs. Then the lattice forming parts will be counted and the titer calculated. The titer of a hemagglutination assay is determined by the last viable "lattice" structure found. This is because it is at the point where, if diluted anymore, the amount of Virus particles will be less than that of the RBCs and thus not be able to agglutinate them together.
Synonyms: Anti-RBC antibody, Red Blood Cell Antibody, Antibody for hemagglutination, rabbit anti RBC, rabbit antibody to sheep Red Blood Cells (RBC), haemolysin, hemolysin, erythrocytes sensitizing agent
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