HSF1 抗体 (N-Term)
Quick Overview for HSF1 抗体 (N-Term) (ABIN7565973)
抗原
See all HSF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- HSF1 Antibody
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交叉反应 （详细）
- Anti-HSF1 is directed against human HSF1 at a n-terminus position.
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产品特性
- Synonyms: rabbit anti-HSF1 antibody, HSF-1, HSF 1, heat shock factor protein 1, HSTF1, HSTF 1, HSTF-1, heat shock transcription factor 1
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纯化方法
- This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Affinity purified Anti-HSF1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the n-terminal portion of human HSF1 protein.
Immunogen Type: Conjugated Peptide
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anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (HRP)
HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 HRP
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (FITC)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 FITC
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Biotin)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Biotin
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PE)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 PE
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (APC)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 APC
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PerCP)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 PerCP
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 488)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 488
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 594)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 594
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 390)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 390
anti-Heat Shock Factor Protein 1 (HSF1) (AA 350-529) antibodyHSF1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Heat Shock Factor 1 Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of 57 kDa in size corresponding to HSF1 by western blotting in the appropriate cell lysate or extract. Tested using positive control A431 Whole Cell Lysate p/n W09-000-361. Western Blot Dilution: 1 μg/mL ELISA Dilution: 5 μg/mL
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.96 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- HSF1 (Heat Shock Factor Protein 1 (HSF1))
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别名
- HSF1
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背景
- Background: Anti-HSF1 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Heat shock factor 1 (HSF1) is a highly conserved transcription factor that coordinates stress-induced transcription and directs versatile physiological processes in eukaryotes. Upon stress, HSF1 is the key transcriptional activator of chaperones, co-chaperones and ubiquitin, and also coordinates the expression of many transcriptional and translational regulators, signaling molecules and mitotic determinants. HSF1 is involved in balancing core cellular processes during stress and enables their rapid re-establishment once conditions suitable for proliferation have been restored. Importantly, HSF1 controls a distinct set of target genes in cell stress, development and cancer progression. The central role of HSF1 in diverse cellular functions is reflected in pathologies, such as neurodegenerative diseases and cancer, where an imbalance in HSF1 activity facilitates disease onset. The ability of cancer cells to harness HSF1 for metastatic progression highlights the plasticity of HSF1 in rewiring transcription and coordinating cellular processes. Anti-HSF1 antibody is thus ideal for investigators involved in cancer research and neurodegenerative diseases.
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基因ID
- 3297
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NCBI登录号
- NP_005517
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UniProt
- Q00613
抗原
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