O3FAR1 抗体 (N-Term)
Quick Overview for O3FAR1 抗体 (N-Term) (ABIN7565972)
抗原
See all O3FAR1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- Ffar4 Antibody
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交叉反应 （详细）
- Anti-Ffar4 is directed against mouse Ffar4 at a n-terminal position.
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产品特性
- Synonyms: rabbit anti-Ffar4 antibody, Ffar-4, Ffar 4, free fatty acid receptor 4, omega-3 fatty acid receptor 1, G-protein coupled receptor 120, G-protein coupled receptor GT01, Gpr120, O3far1
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纯化方法
- This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Affinity purified Anti-Ffar4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the N-terminus portion of mouse Ffar4 protein.
Immunogen Type: Conjugated Peptide
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anti-omega-3 Fatty Acid Receptor 1 (O3FAR1) (N-Term) antibody
O3FAR1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-omega-3 Fatty Acid Receptor 1 (O3FAR1) antibodyO3FAR1 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-omega-3 Fatty Acid Receptor 1 (O3FAR1) (AA 22-36), (Extracellular) antibodyO3FAR1 适用: 小鼠 WB, IHC, FACS, LCI 宿主: 兔 Polyclonal unconjugated
anti-omega-3 Fatty Acid Receptor 1 (O3FAR1) (AA 247-258) antibodyO3FAR1 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-omega-3 Fatty Acid Receptor 1 (O3FAR1) (C-Term) antibodyO3FAR1 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-omega-3 Fatty Acid Receptor 1 (O3FAR1) (Internal Region) antibodyO3FAR1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-omega-3 Fatty Acid Receptor 1 (O3FAR1) (Internal Region) antibodyO3FAR1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-omega-3 Fatty Acid Receptor 1 (O3FAR1) (Internal Region) antibodyVerified O3FAR1 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-omega-3 Fatty Acid Receptor 1 (O3FAR1) (AA 243-292) antibodyO3FAR1 适用: 人, 小鼠, Cow, Pig WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Ffar4 Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~40-48 kDa in size corresponding to Ffar4 by western blotting in the appropriate cell lysate or extract. Tested using positive controls Raw 264.7 WCL p/n W10-001-369 and NIH/3T3 WCL p/n W10-000-358. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 1:50,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- O3FAR1 (omega-3 Fatty Acid Receptor 1 (O3FAR1))
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别名
- Ffar4
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背景
- Background: Anti-Ffar4 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Free Fatty Acid 4 receptor (Ffa4 receptor or GPR120), a rhodopsin-like G protein coupled receptor (GPCR) subfamily member, is a receptor that senses specific fatty acids such as omega-3 fatty acid in fish oil or the endogenous signaling lipid, PHASA. Ffa4 receptor is enriched in lung, colon and adipose tissue but is also detected in many other tissues and cells. The activation of Ffar4 has multiple effects, including but not limited to inhibition of inflammation, improving insulin sensitivity and adipogenesis, and regulating hormone secretion from the gastro-intestinal system and pancreatic islets. Therefore, approaches that regulate FFA4 receptor activity could be developed as promising anti-diabetic and anti-inflammation drugs. GPR120 is the only fatty acid receptor that can sense lipids in adipose tissue, mature adipocytes, CD11c+ macrophages, and RAW264.7 cells making this receptor of potential importance in the prevention and treatment of metabolic and inflammatory diseases.
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基因ID
- 107221
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NCBI登录号
- NP_861413
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UniProt
- Q7TMA4
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途径
- Hormone Transport
抗原
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