EZH1 抗体
Quick Overview for EZH1 抗体 (ABIN7565971)
抗原
See all EZH1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- EZH1 Antibody
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交叉反应 （详细）
- Anti-EZH1 is directed against human EZH1 at an internal position.
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产品特性
- Synonyms: rabbit anti-EZH1 antibody, KIAA0388, Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1, EZH-1, EZH 1
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纯化方法
- This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Affinity purified Anti-EZH1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human EZH1 protein.
Immunogen Type: Conjugated Peptide
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anti-Enhancer of Zeste Homolog 1 (EZH1) (AA 135-294) antibody
EZH1 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 3B6B9 unconjugated
anti-Enhancer of Zeste Homolog 1 (EZH1) (AA 135-294) antibodyEZH1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4B11B4 unconjugated
anti-Enhancer of Zeste Homolog 1 (EZH1) (AA 393-422) antibodyEZH1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enhancer of Zeste Homolog 1 (EZH1) (Internal Region) antibodyEZH1 适用: 人, 小鼠, 猴 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Enhancer of Zeste Homolog 1 (EZH1) (Internal Region) antibodyEZH1 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Enhancer of Zeste Homolog 1 (EZH1) (Internal Region) antibodyEZH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Enhancer of Zeste Homolog 1 (EZH1) (AA 160-280) antibodyEZH1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enhancer of Zeste Homolog 1 (EZH1) (AA 160-280) antibodyEZH1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enhancer of Zeste Homolog 1 (EZH1) (N-Term) antibodyEZH1 适用: 人, 小鼠, 大鼠, 猴, Cow, 豚鼠, 马, 犬, 兔, 斑马鱼, Bat, 小鸡, Pig, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Enhancer of Zeste Homolog 1 (EZH1) (N-Term) antibodyVerified EZH1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
- Application Note: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~86 kDa in size corresponding to Ezh1 by western blotting in the appropriate cell lysate or extract. Tested using positive control NIH/3T3 nuclear extract p/n W10-001-A74. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 1:50,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.95 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- EZH1 (Enhancer of Zeste Homolog 1 (EZH1))
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别名
- EZH1
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背景
- Background: Anti-Ezh1 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Anti-EZH1 Antibody detects human EZH1. Polycomb group (PcG) proteins are essential epigenetic regulators in normal tissue homeostasis and are involved in transcriptional repression. The PcG members Ezh2 and Ezh1 are important determinants of embryonic stem cell identity, and the transcript levels of these histone methyltransferases are inversely correlated during development. Recent studies have shown that EZH1 also has histone H3K27 methyltransferase activity and binds to an overlapping subset of genes. EZH1 and EZH2 have different expression patterns. EZH2 is found in actively proliferating cells, whereas EZH1 expression is higher in nonproliferative adult tissues. EZH1 partially compensates for the loss of EZH2, as shown in cells lacking only Ezh2. In mice, EZH1 is a regulator of homeotic gene expression implicated in the assembly of repressive protein complexes in chromatin. Anti-EZH1 Antibody is ideal for investigators studying pathogenesis of hematological malignancies.
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基因ID
- 2145
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NCBI登录号
- NP_001982
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UniProt
- Q92800
抗原
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