POLR2A/RPB1 抗体 (N-Term)
Quick Overview for POLR2A/RPB1 抗体 (N-Term) (ABIN7565953)
抗原
See all POLR2A/RPB1 (POLR2A) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- POL II Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human POL II.
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产品特性
- Synonyms: RPO2, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RPB1, POLR2A,
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纯化方法
- This affinity purified antibody is directed against human POL II.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-POL II antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminal portion of human POL II conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide
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anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer2) antibody
POLR2A 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibodyPOLR2A 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibodyPOLR2A 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 1-260) antibodyPOLR2A 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 1-260) antibodyPOLR2A 适用: 人 WB, IHC, IF, IP, ChIP 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibodyPOLR2A 适用: 人 WB, IF, ELISA, ChIP, FM 宿主: 小鼠 Monoclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer2) antibodyPOLR2A 适用: 人 WB, IF, ELISA, ChIP, FM 宿主: 小鼠 Monoclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer2) antibodyPOLR2A 适用: 人 WB, IHC, IF, ELISA, IP 宿主: 兔 Monoclonal 2G1 unconjugated Recombinant Antibody
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 128-258) antibodyPOLR2A 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 1B1D8 unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 76-298) antibodyPOLR2A 适用: 人 IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-POL II antibody has been tested in ChIP and ELISA, and is likely suitable for western blotting and immunofluorescence assays. Positive controls used in ChIP were EGF stimulated NR4A1 5', NR4A1 3' and FOS 3'. The negative control was total rabbit IgG. ChIP Dilution: User Optimized Western Blot Dilution: 1:1000 ELISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
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别名
- POLR2A
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背景
- Background: Anti-POL II antibody will detect DNA-directed RNA polymerase II subunit RPB1, an enzyme composed of 12 subunits, which initiates the transcription of RNA from DNA, synthesizing many functional non-coding RNAs as well as messenger RNA precursors. POL II encodes a core element of the transcription machinery. Malfunctioning of the Pol II gene can cause diseases such as Cockayne Syndrome B and La Crosse Encephalitis. Anti-POL II Antibody is useful for researchers interested in DNA damage and repair studies.
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基因ID
- 5430
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NCBI登录号
- NP_000928
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UniProt
- P24928
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途径
- Regulatory RNA Pathways
抗原
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