CH25H 抗体 (C-Term)
Quick Overview for CH25H 抗体 (C-Term) (ABIN7565948)
抗原
See all CH25H 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- CH25H Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human CH25H.
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产品特性
- Synonyms: Rabbit Anti-Cholesterol 25-Hydroxylase, Rabbit Anti-CH25H, Cholesterol 25-Monooxygenase, EC 1.14.99.38, H25OH, C25H
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纯化方法
- This affinity purified antibody is directed against human CH25H.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-CH25H antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of human CH25H conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide
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anti-Cholesterol 25-Hydroxylase (CH25H) (AA 1-100) antibody
CH25H 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Cholesterol 25-Hydroxylase (CH25H) (AA 142-272) antibodyCH25H 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cholesterol 25-Hydroxylase (CH25H) (AA 206-272) antibodyCH25H 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Cholesterol 25-Hydroxylase (CH25H) (AA 190-239) antibodyCH25H 适用: 人, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cholesterol 25-Hydroxylase (CH25H) (AA 47-76) antibodyCH25H 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB55304 unconjugated
anti-Cholesterol 25-Hydroxylase (CH25H) (AA 142-247) antibodyCH25H 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G8 unconjugated
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应用备注
- Application Note: Anti-CH25H Antibody has been tested in ELISA, WB, and IHC. Expect a band at ~31.7 kDa in western blot using appropriate tissues or lysates. Positive control used: Human Kidney Tissues in Immunohistochemistry. Immunohistochemistry Dilution: 1:100 Western Blot Dilution: 1:1,000 ELISA Dilution: 1:10,000 - 1:50,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.92 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CH25H (Cholesterol 25-Hydroxylase (CH25H))
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别名
- CH25H
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背景
- Background: CH25H (Cholesterol 25-Hydroxylase catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. CH25H plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for several lymphoid cells. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. Anti-CH25H Antibody is useful for researchers interested in Alzheimer's Disease, Neuroscience, and metabolism.
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基因ID
- 9023
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NCBI登录号
- NP_003947
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UniProt
- O95992
抗原
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