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CH25H 抗体 (AA 1-100)

Name: CH25H 抗体 (AA 1-100)
SKU: ABIN1387938
Availability: OutOfStock

CH25H 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-CH25H Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining. (CH25H 抗体 (AA 1-100))

IHC (p)

1 image

(1 reference)

产品编号 ABIN1387938

发货至: 中国

Datasheet as PDF

抗原 See all CH25H 抗体

CH25H (Cholesterol 25-Hydroxylase (CH25H))
抗原表位
14

5

1

1

1

1

1
AA 1-100
适用
16

12

1

1

1

1

1

1

1

1

1
小鼠
宿主
23

3
兔
Compatible Secondaries
克隆类型
24

2
多克隆
标记
8

3

2

2

1

1

1

1

1

1

1

1

1

1

1
This CH25H antibody is un-conjugated
应用范围
21

6

4

2

2

1
Western Blotting (WB)

交叉反应

人

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human CH25H

亚型

IgG
anti-Cholesterol 25-Hydroxylase (CH25H) (AA 142-272) antibody
CH25H 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Cholesterol 25-Hydroxylase (CH25H) (AA 206-272) antibody
CH25H 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Cholesterol 25-Hydroxylase (CH25H) (AA 190-239) antibody
CH25H 适用: 人, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Cholesterol 25-Hydroxylase (CH25H) (AA 47-76) antibody
CH25H 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB55304 unconjugated

anti-Cholesterol 25-Hydroxylase (CH25H) (AA 142-247) antibody
CH25H 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G8 unconjugated

anti-Cholesterol 25-Hydroxylase (CH25H) (AA 1-100) antibody (Biotin)
CH25H 适用: 小鼠 WB 宿主: 兔 Polyclonal Biotin

anti-Cholesterol 25-Hydroxylase (CH25H) (AA 1-100) antibody (Alexa Fluor 350)
CH25H 适用: 小鼠 WB 宿主: 兔 Polyclonal Alexa Fluor 350

anti-Cholesterol 25-Hydroxylase (CH25H) (AA 1-100) antibody (Alexa Fluor 488)
CH25H 适用: 小鼠 WB 宿主: 兔 Polyclonal Alexa Fluor 488

anti-Cholesterol 25-Hydroxylase (CH25H) (AA 1-100) antibody (Alexa Fluor 555)
CH25H 适用: 小鼠 WB 宿主: 兔 Polyclonal Alexa Fluor 555

anti-Cholesterol 25-Hydroxylase (CH25H) (AA 1-100) antibody (Alexa Fluor 647)
CH25H 适用: 小鼠 WB 宿主: 兔 Polyclonal Alexa Fluor 647

应用备注

ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Mukherjee, Hough, Chattopadhyay, Navab, Fogelman, Meriwether, Williams, Bensinger, Moller, Faull, Lusis, Iruela-Arispe, Bostrom, Tontonoz, Reddy, Fogelman: "Transgenic tomatoes expressing the 6F peptide and ezetimibe prevent diet-induced increases of IFN-β and cholesterol 25-hydroxylase in jejunum." in: Journal of lipid research, Vol. 58, Issue 8, pp. 1636-1647, (2017) (PubMed).
抗原

CH25H (Cholesterol 25-Hydroxylase (CH25H))

别名

CH25H (CH25H 产品)

别名

CH25H antibody, zgc:101688 antibody, MGC143045 antibody, C25H antibody, AI462618 antibody, m25OH antibody, cholesterol 25-hydroxylase antibody, cholesterol 25-hydroxylase L homeolog antibody, CH25H antibody, ch25h antibody, Ch25h antibody, ch25h.L antibody

背景

Synonyms: C25H, Cholesterol 25-hydroxylase, Cholesterol 25-monooxygenase, h25OH, CH25H
Background: Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.

基因ID

9023

UniProt

O95992