SMAD3 抗体 (Internal Region)
Quick Overview for SMAD3 抗体 (Internal Region) (ABIN7565933)
抗原
See all SMAD3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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原理
- Ready-To-Use SMAD3 Antibody
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交叉反应 （详细）
- RTU Anti-SMAD3 Antibody is directed against human Smad3 protein.
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产品特性
- Synonyms: hMAD 3 antibody, hSMAD3 antibody, MADH3 antibody, MGC60396 antibody, Mothers against decapentaplegic homolog 3 antibody, Mothers against DPP homolog 3 antibody, Smad-3
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: SMAD3 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.
Immunogen Type: Conjugated Peptide
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anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 适用: 人 WB, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 7F3 unconjugated
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (pSer423), (pSer425) antibodySMAD3 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-230) antibodySMAD3 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibodySMAD3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2C12 unconjugated
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-110) antibodySMAD3 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1C11 unconjugated
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibodySMAD3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C12 unconjugated
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) antibodySMAD3 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS, IF (cc) 宿主: 兔 Monoclonal 3D1 unconjugated
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 231-425) antibodySMAD3 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (C-Term) antibodySMAD3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) antibodyKO Validated SMAD3 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
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应用备注
- Application Note: Ready-To-Use Anti-SMAD3 Antibody has been optimized and validated in western blot using 1:1000 dilution. This Anti-SMAD3 (RTU) Antibody is sufficient to run 10 western blots. Expect a band approximately 48.1 kDa in size corresponding to human Smad3 protein by western blotting in the appropriate tissue or cell lysate or extract. Although not tested, this antibody is likely functional in ELISA, immunoprecipitation, and immunohistochemistry. Optimal titers for these applications should be determined by the researcher. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:1,000 Immunoprecipitation Dilution: User Optimized ELISA Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.01 % Bovine Serum Albumin (BSA), 25 % (v/v) Glycerol
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 2-8° C prior to opening. May aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to use.
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有效期
- 12 months
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- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
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别名
- SMAD3
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the S-S-X-S motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the bone morphogenic protein or BMP pathway. Upon phosphorylation R-Smads are translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80 % of all TGF-b-mediated response. All Rockland Immunochemical's SMAD3 antibodies are affinity-purified to assure both high affinity and specificity. Rigorous quality control testing ensures that the finished product meets or exceeds out high standards for optimum performance in your assays.
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基因ID
- 4088
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NCBI登录号
- NP_005893
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UniProt
- P84022
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途径
- Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
抗原
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