PGC 抗体 (HRP)
Quick Overview for PGC 抗体 (HRP) (ABIN7565906)
抗原
See all PGC 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Phospho Enol Pyruvate Carboxylase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate Carboxylase [Maize].
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产品特性
- Synonyms: rabbit anti-Phospho Enol Pyruvate Carboxylase Antibody Peroxidase Conjugation, PE conjugated rabbit anti-PEPC 1 antibody, PEPCase 1 antibody, Phosphoenolpyruvate carboxylase 1 antibody
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纯化方法
- Phospho Enol Pyruvate Carboxylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Phospho-enol-pyruvate Carboxylase [Maize Leaves]
Immunogen Type: Native Protein
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anti-Pepsinogen C (PGC) (AA 100-200) antibody
PGC 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pepsinogen C (PGC) (AA 17-392) antibodyPGC 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pepsinogen C (PGC) (Internal Region) antibodyPGC 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pepsinogen C (PGC) (AA 61-388) antibodyPGC 适用: 人 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Pepsinogen C (PGC) (AA 1-388) antibodyPGC 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Pepsinogen C (PGC) (AA 1-388) antibodyPGC 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Pepsinogen C (PGC) (AA 17-392) antibodyPGC 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pepsinogen C (PGC) (AA 21-120) antibodyPGC 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Pepsinogen C (PGC) (pSer15) antibodyPGC 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Pepsinogen C (PGC) (AA 17-388) antibodyPGC 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Phosphoenolpyruvate Carboxylase Peroxidase conjugated antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Phospho-enol-Pyruvate Carboxylase [Maize] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- PGC (Pepsinogen C (PGC))
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别名
- pepc
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背景
- Background: Phospho Enol Pyruvate Carboxylase is an enzyme in the family of carboxy-lyases that catalyzes the addition of bicarbonate to phosphoenolpyruvate (PEP) to form a four-carbon compound oxaloacetate. This reaction is used for carbon fixation in CAM and C4 plants where it plays a key role in photosynthesis. The enzyme is also found in some bacteria, but not in animals or fungi.
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基因ID
- 542372
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UniProt
- B8XPZ2
抗原
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