DNASE1 抗体 (HRP)
Quick Overview for DNASE1 抗体 (HRP) (ABIN7565874)
抗原
See all DNASE1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Deoxyribonuclease I Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease I [Bovine Pancreas].
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产品特性
- Synonyms: rabbit anti-Deoxyribonuclease I Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Deoxyribonuclease I Antibody, Deoxyribonuclease-1, Deoxyribonuclease I, DNase I
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纯化方法
- Deoxyribonuclease I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Deoxyribonuclease I [Bovine Pancreas]
Immunogen Type: Native Protein
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anti-Deoxyribonuclease I (DNASE1) (AA 19-259) antibody
DNASE1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 22-282) antibodyDNASE1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) antibodyDNASE1 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 1-252) antibodyDNASE1 适用: 人 WB, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 19-259) antibodyDNASE1 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal 2-1# unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 78-231) antibodyDNASE1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 87-121) antibodyDNASE1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB49355 unconjugated
anti-Deoxyribonuclease I (DNASE1) antibodyDNASE1 适用: 大鼠, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (N-Term) antibodyDNASE1 适用: 人, 大鼠, Cow WB 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 19-259) antibodyDNASE1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 7# unconjugated
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应用备注
- Application Note: Anti-Deoxyribonuclease I Peroxidase Conjugated Antibody has been tested by ELISA and Western blot. This product is assayed against 1.0 μg of Deoxyribonuclease I [Bovine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:5,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- DNASE1 (Deoxyribonuclease I (DNASE1))
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别名
- DNASE1, DNL1
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背景
- Background: Deoxyribonuclease I (DNase I) is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin.
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基因ID
- 282217
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NCBI登录号
- NP_776959
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UniProt
- P00639
抗原
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