PIAS1 抗体 (Internal Region)
Quick Overview for PIAS1 抗体 (Internal Region) (ABIN7565807)
抗原
See all PIAS1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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原理
- PIAS1 Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest reactivity to mouse 92.
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产品特性
- Synonyms: Rabbit anti-PIAS1 Antibody, Protein Inhibitor Of Activated STAT 1, GBP, ZMIZ3, DDXBP1, DEAD/H (Asp-Glu-Ala-Asp/His) Box Binding Protein 1, GU/RH-II, E3 SUMO-protein ligase PIAS1, Gu-binding protein, GBP, AR Interacting Protein
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纯化方法
- Anti-PIAS1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-PIAS1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide from the internal region of human PIAS1.
Immunogen Type: Conjugated Peptide
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anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 636-651), (C-Term) antibody
PIAS1 适用: 人, 小鼠, 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 636-651) antibodyPIAS1 适用: 人, 小鼠, 大鼠 WB, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (N-Term) antibodyPIAS1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 422-651) antibodyPIAS1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 543-651) antibodyPIAS1 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 4A4 unconjugated
anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 422-651) antibodyPIAS1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) antibodyPIAS1 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (N-Term) antibodyPIAS1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 409-645) antibodyPIAS1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 1-651) antibodyPIAS1 适用: 人 IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-PIAS1 Antibody has been tested for use in ELISA, Western Blotting, and Dot Blotting. HEK293 WCL p/n (W09-000-365) and K-562 WCL p/n (W09-001-GJ7) were used as positive control lysates in western blot. Expect a band at approximately 72 kDa in Western Blots of specific cell lysates and tissues. Specific conditions for reactivity should be optimized by the end user. While not tested, it is likely Anti-PIAS1 antibody will work for immunofluorescence, IHC, and CHIP assays. Western Blot Dilution: 1:1000 ELISA Dilution: 5 μg/mL IF Microscopy Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))
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别名
- PIAS1
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背景
- Background: The PIAS proteins (protein inhibitor of activated STAT) play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS1. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS1 binds specifically to STAT1, inhibiting STAT1-mediated gene activation and also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS1 is a potent co-activator for CP2c-mediated alpha-globin expression in erythroid cells. Anti-PIAS1 Antibody is useful for researchers interested in epigenetics and cancer research.
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基因ID
- 8554
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NCBI登录号
- NP_057250
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UniProt
- O75925
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途径
- JAK/STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C
抗原
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