GATA4 抗体
Quick Overview for GATA4 抗体 (ABIN7565766)
抗原
See all GATA4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- GATA4 Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest reactivity with mouse and rat GATA4 based on a 93 % homology.
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产品特性
- Synonyms: mouse anti-GATA4 Antibody, Transcription factor GATA-4, GATA-binding factor 4, GATA-4 Antibody
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纯化方法
- Anti-GATA4 was purified from concentrated tissue culture supernatant Protein A chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to the first 100 amino acids of human GATA4 protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG1 kappa
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anti-GATA Binding Protein 4 (GATA4) (pSer105) antibody
GATA4 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-GATA Binding Protein 4 (GATA4) (pSer262) antibodyGATA4 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-GATA Binding Protein 4 (GATA4) antibodyGATA4 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-GATA Binding Protein 4 (GATA4) (AA 228-440) antibodyGATA4 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-GATA Binding Protein 4 (GATA4) (AA 50-150) antibodyGATA4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-GATA Binding Protein 4 (GATA4) (AA 261-360) antibodyGATA4 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-GATA Binding Protein 4 (GATA4) (AA 201-442) antibodyGATA4 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-GATA Binding Protein 4 (GATA4) (AA 330-380) antibodyGATA4 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-GATA Binding Protein 4 (GATA4) (AA 343-442) antibodyGATA4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6C6 unconjugated
anti-GATA Binding Protein 4 (GATA4) (AA 296-442) antibodyGATA4 适用: 人 WB, IF 宿主: 小鼠 Monoclonal 1H8 unconjugated
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应用备注
- Application Note: Anti-GATA4 antibody has been tested by ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44 kDa in size corresponding to GATA4 by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1:500-1:1000 ELISA Dilution: 1:2000-1:10,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- GATA4 (GATA Binding Protein 4 (GATA4))
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别名
- GATA4
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背景
- Background: GATA4, also know as GATA-binding factor 4, acts in cooperation with NKX2-5 as a transcriptional activator of ANF. It binds to the consensus sequence 5'-AGATAG-3' on DNA. GATA4 is critical for the enlargement of cardiac myocytes. Mutations in GATA4 have been linked with congenital heart malfunctions and malformations. Anti-GATA4 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.
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基因ID
- 2626
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NCBI登录号
- NP_002043
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UniProt
- P43694
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途径
- Peptide Hormone Metabolism, Carbohydrate Homeostasis
抗原
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