Biotin 抗体 (FITC)
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北京 101111
Quick Overview for Biotin 抗体 (FITC) (ABIN7565751)
抗原
宿主
克隆类型
标记
应用范围
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原理
- Biotin Antibody Fluorescein Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Albumin.
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产品特性
- Synonyms: rabbit anti-Biotin Antibody FITC Conjugation, Fluorescein conjugated rabbit anti-Biotin antibody, Biotin FITC, Anti-Biotin fluorescein Antibody
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纯化方法
- Anti-Biotin Antibody Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Biotin conjugated Keyhole Limpet Hemocyanin (KLH)
Immunogen Type: Native Protein
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Labeling Ratio
- 2.7
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anti-Biotin antibody
WB, IHC, IP, ICC 宿主: 兔 Monoclonal BLR315M unconjugated Recombinant Antibody
anti-Biotin antibodyWB, IHC, IP, ICC 宿主: 兔 Monoclonal BLR315M unconjugated Recombinant Antibody
anti-Biotin antibodyWB, IHC, ICC 宿主: 兔 Monoclonal BLR328M unconjugated Recombinant Antibody
anti-Biotin antibodyWB, IHC, ELISA, FACS, IF, FM 宿主: 小鼠 Monoclonal 4C7-D5 unconjugated
anti-Biotin antibody适用: Various Species IHC 宿主: 兔 Monoclonal BTN-2032R unconjugated Recombinant Antibody
anti-Biotin antibody适用: Various Species WB, IHC, FACS, IF 宿主: 小鼠 Monoclonal Hyb-8 unconjugated
anti-Biotin antibodyWB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Biotin antibody (DyLight 488)WB, FACS, IF, FM, FLISA, DB, MA 宿主: 山羊 Polyclonal DyLight 488
anti-Biotin antibody适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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应用备注
- Application Note: Anti-Biotin Antibody Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: User Optimized IF Microscopy Dilution: 1:50:1:300 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Biotin
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物质类
- Chemical
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背景
- Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Fluorescein Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
抗原
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