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AIF 抗体 (AA 131-230)

Name: AIF 抗体 (AA 131-230)
SKU: ABIN724010
Availability: OutOfStock

This anti-AIF antibody is a 兔多克隆 antibody detecting AIF in WB, ELISA, ICC, IHC (p), IF (cc), IF (p) 和 IHC (fro). Suitable for 人, 小鼠, 大鼠和 Pig. This Primary Antibody has been cited in 2+ publications.

Mouse Liver lysates probed with AIF Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C. (AIF 抗体 (AA 131-230))

Formalin-fixed and paraffin embedded labeled with human gastric carcinoma Rabbit Anti-AIF Polyclonal Antibody, Unconjugated (ABIN724010) 1:300 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE (Negative Control) (AIF 抗体 (AA 131-230))

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AIF Polyclonal Antibody, Unconjugated (ABIN724010) 1:300 followed by conjugation to the secondary antibody and DAB staining (AIF 抗体 (AA 131-230))

IHC

Formalin-fixed and paraffin embedded human cervical tissue labeled with Anti-AIF Polyclonal Antibody, Unconjugated (ABIN724010), followed by conjugation to the secondary antibody and DAB staining (AIF 抗体 (AA 131-230))

IHC

K562 lysates probed with AIF Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C. (AIF 抗体 (AA 131-230))

HT1080 lysates probed with Anti-AIF Polyclonal Antibody at 1:5000 for 90 min at 37˚C. (AIF 抗体 (AA 131-230))

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AIF Polyclonal Antibody, Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining (AIF 抗体 (AA 131-230))

IHC (p)

Formalin-fixed and paraffin embedded labeled with human gastric carcinoma Rabbit Anti-AIF Polyclonal Antibody, Unconjugated 1:300 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE (Negative Control) (AIF 抗体 (AA 131-230))

IF (p)

10 images

(2 references)

产品编号 ABIN724010

发货至: 中国

Datasheet as PDF

Quick Overview for AIF 抗体 (AA 131-230) (ABIN724010)

抗原

See all AIF (AIFM1) 抗体

AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

适用

人, 小鼠, 大鼠, Pig

宿主

兔

克隆类型

多克隆

标记

This AIF antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

抗原表位
14

10

9

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6

4

4

4

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 131-230

交叉反应

人, 小鼠, Pig, 大鼠

预测反应

Dog,Cow,Sheep,Chicken,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human AIF

亚型

IgG
anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) antibody
AIFM1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 4E7 unconjugated

anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 103-322) antibody
AIFM1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody
Verified AIFM1 适用: 人, 小鼠, 大鼠, Pig WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated

anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 334-613) antibody
AIFM1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 183-195) antibody
Verified AIFM1 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated

anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 103-612) antibody
AIFM1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 1-613) antibody
AIFM1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 1-613) antibody
AIFM1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody
AIFM1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (N-Term) antibody
AIFM1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Li, Chen, Lu, Zhang, Guan, Li, Wang: "Anti-Oxidative Stress Activity Is Essential for Amanita caesarea Mediated Neuroprotection on Glutamate-Induced Apoptotic HT22 Cells and an Alzheimer's Disease Mouse Model." in: International journal of molecular sciences, Vol. 18, Issue 8, (2017) (PubMed).

Gong, Hua, Zhang, Zhao, Tang, Mei, Zhang, Cui, Li: "Hypothermia-induced neuroprotection is associated with reduced mitochondrial membrane permeability in a swine model of cardiac arrest." in: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, Vol. 33, Issue 6, pp. 928-34, (2013) (PubMed).
抗原

AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

别名

AIF

背景

Synonyms: AIF, CMT2D, CMTX4, COWCK, NADMR, NAMSD, PDCD8, COXPD6, Apoptosis-inducing factor 1, mitochondrial, Programmed cell death protein 8, AIFM1
Background: Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.

基因ID

9131

UniProt

O95831

途径

Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect