Recombinant CD24 抗体
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北京 101111
Quick Overview for Recombinant CD24 抗体 (ABIN7543435)
抗原
See all CD24 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-CD24 [M1/69], Rabbit IgG, kappa
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特异性
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This antibody binds mouse CD24 also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a variably glycosylated GPI-anchored membrane protein expressed on the surface lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. This protein plays an important role in differentiation of different cell types and its expression varies during lymphocyte differentiation which makes it a good marker for delineating various lymphocyte developmental stages. It is also expressed in erythroleukemia, small cell lung carcinoma and multiple sclerosis.
Application Notes original Clone: This antibody was capable of recognizing CD24 antigen from microsomal membrane preparation of different mouse cells through western blot (PMID: 1696896). This antibody is recommended for immunophenotypic analysis of CD24 expressed on mouse B and T cells using flow cytometry (PMID: 17702899, 20133615, 20203098, 33111080). This antibody (dilution 1:100) was also used in the immunohistochemical analysis of CD24 expression in mouse thyroid cells using 5-μm zinc-fixed, paraffin-embedded thyroid sections (PMID: 18801910). This antibody was also reported to be used in the flow cytometric and immunohistochemical analysis of murine CD24 expressed on the surface of normal human breast epithelial cells early after their infection with a retroviral vector (PMID: 9232274). This antibody was also used in the immunofluorescence staining of murine spinal cord sections (PMID: 17475850).
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产品特性
- This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing DA rats with B10 mouse splenic T cells and concanavalin A-activated splenocytes.
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亚型
- IgG, kappa
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anti-CD24 Molecule (CD24) (AA 15-80) antibody
CD24 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 4F4E10 unconjugated
anti-CD24 Molecule (CD24) antibody (FITC)CD24 适用: 人 FACS 宿主: 小鼠 Monoclonal SN3 FITC
anti-CD24 Molecule (CD24) (AA 60-80) antibodyCD24 适用: 人, 小鼠, 大鼠 IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD24 Molecule (CD24) (AA 1-80) antibodyCD24 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD24 Molecule (CD24) antibody (APC)CD24 适用: 人 FACS 宿主: 小鼠 Monoclonal SN3 APC
anti-CD24 Molecule (CD24) antibodyCD24 适用: 人 FACS, IHC (fro), MS 宿主: 小鼠 Monoclonal SN3 unconjugated
anti-CD24 Molecule (CD24) antibody (PE)CD24 适用: 人 FACS 宿主: 小鼠 Monoclonal SN3 PE
anti-CD24 Molecule (CD24) (AA 15-80) antibodyCD24 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-CD24 Molecule (CD24) antibodyCD24 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C4 unconjugated
anti-CD24 Molecule (CD24) antibodyCD24 适用: 人, Chimpanzee FACS, IHC (fro) 宿主: 小鼠 Monoclonal SN3 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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OriginalSpeciesName: Rat
OriginalFormat: IgG2b
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- CD24 (CD24 Molecule (CD24))
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别名
- CD24
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背景
- Heat stable antigen, Signal transducer CD24, Lymphocyte antigen 52, Ly-52, M1/69-J11D heat stable antigen, HSA, Nectadrin, R13-Ag, X62 heat stable antigen
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UniProt
- P24807
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
抗原
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