Recombinant Nerve Growth Factor 抗体
Quick Overview for Recombinant Nerve Growth Factor 抗体 (ABIN7543390)
抗原
See all Nerve Growth Factor (NGF) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Anti-Nerve growth factor [mab 911], Rabbit IgG, kappa
-
特异性
- The antibody is specific for NGF. The NGF epitope is composed of β-strand segments from both NGF monomers. NGF is involved primarily in the growth, as well as the maintenance, proliferation, and survival of nerve cells (neurons).
-
交叉反应
- 小鼠
-
产品特性
-
Original Species of Ab: Mouse
Original Format of Ab: IgG1
-
纯化方法
- Protein A affinity purified
-
亚型
- IgG kappa
-
-
anti-Nerve Growth Factor (NGF) antibody
NGF 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) (AA 19-241) antibodyNGF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) (AA 162-173), (N-Term) antibodyNGF 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) antibodyNGF 适用: 人, 大鼠, 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) (AA 122-218) antibodyNGF 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) (AA 122-241) antibodyNGF 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) antibodyNGF 适用: 人 WB, IHC, ELISA 宿主: 兔 Monoclonal 5F10 unconjugated Recombinant Antibody
anti-Nerve Growth Factor (NGF) (N-Term) antibodyNGF 适用: 人, 大鼠, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) antibody (Biotin)NGF 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
anti-Nerve Growth Factor (NGF) (AA 19-241) antibodyNGF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- The specificity of the original format of the antibody to NGF was confirmed by ELISA analysis (EC50 = 0.37 nM). The antibody was evaluated for its ability to block the binding of hNGF to the TrkA and p75 NGF receptors in various in vitro assays, such as blocking of TrkA autophosphorylation and blocking of NGF-dependent survival of dorsal root ganglion sensory neurons. The antibody was a potent blocker of all activities (Hongo et al., 2000, PMID: 10952410). In vivo administration of the antibody significantly reduces bone cancer pain behaviors (Sevcik et al., 2005, PMID: 15836976 and Halvorson et al., 2005, PMID: 16230406 and Buehlmann et al., 2019, PMID: 30161041) and fracture pain-related behaviors (Koewler et al., 2007, PMID: 17638576). The structure of Nerve Growth factor in complex with the Fab fragment was determined. Library Scanning Mutagenesis method was used to convert the original antibody in the humanized antibody tanezumab. The antibody bound human and murine NGF with high affinity (KD ~10 nM). Tanezumab and the original antibody blocked both TrkA and p75NTR binding to NGF and inhibited NGF-dependent neuron survival. Tanezumab inhibited NGFdependent survival with an IC50 of 15 pM, under the same conditions, 911 inhibited NGF with an IC50 of 400 pM (La Porte et al., 2014, PMID: 24830649). Preventative and therapeutic treatment with the antibody significantly prevented, or reversed, MIA-induced pain behaviour in osteoarthritis (Xu et al., 2016, PMID: 27208420).
-
说明
-
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
-
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- PBS with 0.02 % Proclin 300.
-
储存液
- ProClin
-
注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
-
-
- Nerve Growth Factor (NGF)
-
别名
- Nerve growth factor
-
背景
- Beta-nerve growth factor, Beta-NGF
-
UniProt
- P01138
-
途径
- Regulation of Cell Size
抗原
-
