Recombinant FAP 抗体
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北京 101111
Quick Overview for Recombinant FAP 抗体 (ABIN7543357)
抗原
See all FAP 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-FAP [73.3], Mouse IgG1, kappa
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特异性
- The antibody is specific for fibroblast activation protein (FAP). FAP is a membrane protease that is highly expressed by cancer-associated fibroblasts (CAFs).
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing FAP-null mice with FAP-expressing 3T3 cells.
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亚型
- IgG1 kappa
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anti-Fibroblast Activation Protein, alpha (FAP) (AA 525-624) antibody
FAP 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 1E5 unconjugated
anti-Fibroblast Activation Protein, alpha (FAP) (AA 26-264) antibodyFAP 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 1H12F2 unconjugated
anti-Fibroblast Activation Protein, alpha (FAP) (AA 542-761) antibodyFAP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibroblast Activation Protein, alpha (FAP) (AA 542-761) antibodyFAP 适用: 小鼠 WB, IHC, IP, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibroblast Activation Protein, alpha (FAP) (AA 311-410) antibodyFAP 适用: 人, 小鼠, Cow WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fibroblast Activation Protein, alpha (FAP) (AA 523-760) antibodyFAP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibroblast Activation Protein, alpha (FAP) (AA 523-760) antibodyFAP 适用: 人 WB 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Fibroblast Activation Protein, alpha (FAP) (Internal Region) antibodyFAP 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Fibroblast Activation Protein, alpha (FAP) antibodyFAP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Fibroblast Activation Protein, alpha (FAP) (AA 15-41), (N-Term) antibodyFAP 适用: 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB40867 unconjugated
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应用备注
- A second-generation retroviral CAR, consisting of the scFv fragment of the antibody, with CD8 stalk, human CD3ζ and 4-1BB domains, targeting mouse FAP was developed. The Fap-CAR-T cells killed FAP-expressing 3T3 target cells specifically. The FAP-CAR-T cells were tested in vivo in three different established models, showing that the growth of tumors was significantly reduced by 35-50 % following treatment. Further, the anti-FAP-CAR T cells demonstrated enhanced antitumor response when combined with a tumor vaccine. No significant toxicity was observed in the treated mice. (Wang et al., 2014, PMID: 24778279) FAP+ cells were identified using flow cytometry of lung tissue using the antibody (Kimura et al., 2019, PMID: 31188013). This antibody was used for detection of murine FAP expression on HT1080 cells by flow cytometry (de Sostoa et al. 2019, PMID: 30683154). Immunoprecipitation of FAP was performed on lung humogenate using this antibody. The antibody detected FAP by western blot analysis (Fan et al., 2016, PMID: 26663085).
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- FAP (Fibroblast Activation Protein, alpha (FAP))
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别名
- FAP
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背景
- Fibroblast activation protein, Fibroblast activation protein-α, Prolyl endopeptidase FAP, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, FAPalpha1, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme,Serine integral membrane protease, SIMP, Surface-expressed protease, Seprase
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UniProt
- P97321
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途径
- Tube Formation
抗原
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