Recombinant CD28 抗体
Quick Overview for Recombinant CD28 抗体 (ABIN7543356)
抗原
See all CD28 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-CD28 [9.3], Rabbit IgG, kappa
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特异性
- The antibody is specific for human CD28. CD28 is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG2a
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing mice with human T cells.
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亚型
- IgG kappa
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anti-CD28 (CD28) (AA 1-120) antibody
CD28 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人, Non-Human Primate FACS, WB, IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) antibody (FITC)CD28 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal CD28-2 FITC
anti-CD28 (CD28) antibodyCD28 适用: 人, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) (AA 19-152) antibodyCD28 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM61 unconjugated Recombinant Antibody
anti-CD28 (CD28) (Internal Region) antibodyVerified CD28 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 大鼠 FACS, IP 宿主: 小鼠 Monoclonal JJ319 unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人 FACS, IF, Func 宿主: 小鼠 Monoclonal CB28 unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人, 小鼠, Cow, Pig, 绵羊 FACS, IF, Func 宿主: 小鼠 Monoclonal 204-12 unconjugated
anti-CD28 (CD28) (C-Term) antibodyCD28 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- The antibody was found to induce T cell activation and proliferation in collaboration with TPA. Further, T cell proliferation was associated with IL-2 receptor expression and IL-2 production. The antibody stained 60-80 % of the isolated T cells by immunofluorescence. The antibody immunoprecipitated CD28 from Jurkat cells. (Hara et al., 1985, PMID: 3159820). Immunofluorescence was performed on T cells expressing CD28 using this antibody. The antibody was cytotoxic for 50-80 % of peripheral T cells and for 20-50 % of thymocytes but it was not cytotoxic for cultured B-cell lines, normal B cells, or monocytes. In immune precipitation assays, the antibody reacted with CD28 (Hansen et al., 1980). The antibody induces proliferative responses in human T cells in in vitro experiments (Hutloff et al. 1999, PMID: 9930702). The antibody detected CD28 by western blot analysis (Siefken et al,1998, PMID: 9712026). Humanize, chimeric and hybrid Fab versions of the antibody were constructed. The ability of the three Fabs to bind to CD28 was examined by ELISA, the three Fabs showed a binding activity, with an EC50 of 630, 20 and 30 nM respectively for the humanized, the chimeric, hybrid Fab version. Thus, the humanized Fab showed a moderate loss in avidity. The three Fab versions of the antibody were tested for blocking ligation of CD28-Ig to CHO cells expressing CD80 and the binding was quantified by flow cytometry. The chimeric Fab exhibited the best blocking activity followed by the hybrid and the humanized Fab, with EC50 values of 0.2, 0.4, and 1.2 μM, respectively. Lastly, three Fab versions showed biological activity as an immunosuppressant by inhibiting a MLR (Tan et al. 2002, PMID: 12097421).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- CD28
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别名
- CD28
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背景
- T-cell-specific surface glycoprotein CD28, TP44
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UniProt
- P10747
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
抗原
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