Recombinant KRT14 抗体 (AA 458-472)
Quick Overview for Recombinant KRT14 抗体 (AA 458-472) (ABIN7543316)
抗原
See all KRT14 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 458-472
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原理
- Anti-Cytokeratin 14 [2G4], Rabbit IgG, kappa
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特异性
- This antibody is capable of binding human cytokeratin 14. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing 4-6 weeks old female ICR mice with recombinant protein comprising amino acids 458-472 of cytokeratin 7 in equal volume of Freund's incomplete adjuvant.
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亚型
- IgG kappa
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应用备注
- The binding characterization of this antibody was done using ELISA. The original IgG1 antibody binds cytokeratin 5/6 with a high affinity constant of 4.8x 10^(8) M^(-1). This antibody was also used in the immunohistochemical analysis of frozen paraffin sections of tonsil tissues and esophageal squamous cell carcinoma. The immunohistochemical staining pattern of this antibody is similar to commercially available antibody L1002 (CN113087793).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- KRT14 (Keratin 14 (KRT14))
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别名
- Cytokeratin 14
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背景
- CK14, CK-14, CK 14, K14, KRT14, keratin type I cytoskeletal 14, cytokeratin-14, keratin-14, CGMCC NO 20761, L1002
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UniProt
- P02533
抗原
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