Recombinant F2RL1 抗体 (Cleavage Site)
Quick Overview for Recombinant F2RL1 抗体 (Cleavage Site) (ABIN7543274)
抗原
See all F2RL1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Cleavage Site
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原理
- Anti-PAR2 [Ab4999 (Par-B)], Rabbit IgG, lambda
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特异性
- This antibody has binding specificity for N-terminal PAR-2 peptide 'SKGRSLIGKVDGTSHVTGKGV' and can bind human, mouse, rat and cynomolgus monkey PAR-2 peptide. PAR2 acts as a receptor for trypsin and trypsin-like enzymes coupled to G proteins.
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交叉反应
- Cynomolgus, 小鼠, 大鼠
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产品特性
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Original Species of Ab: Human
Original Format of Ab: IgG4
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated using phage display techniques to pan for antibodies (Fab fragments) capable of immunospecifically binding to residues spanning the protease cleavage site at the N-terminal of PAR-2. The affinity maturation of the selected parental clone Ab3777 resulted in generation of the antibody.
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亚型
- IgG lambda
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anti-Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1) (AA 368-382), (Intracellular) antibody
F2RL1 适用: 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1) (C-Term) antibodyF2RL1 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1) antibodyF2RL1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1) (AA 348-397) antibodyF2RL1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1) (AA 218-399) antibodyF2RL1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1) (N-Term) antibodyF2RL1 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1) (Extracellular Domain) antibodyF2RL1 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1) (N-Term) antibodyF2RL1 适用: 人 ELISA, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal SAM11 unconjugated
anti-Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1) (C-Term) antibodyF2RL1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1) (AA 260-309) antibodyF2RL1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- This antibody was generated by the affinity maturation of a parental clone Ab3777. This antibody exhibited concentration-dependent binding to human PAR-2 transfected HEK293 cells with EC50 values of 0.2 nM. ELISA experiments demonstrated specific binding of this antibody to PAR-2 peptide (coupled to Transferrin as carrier). This antibody did not show any binding affinity for PAR-4 peptide or PAR-1 peptide. This antibody showed potent inhibition of calcium release in the human lung epithelial cell line A549 stimulated with trypsin similar to the parental clone Ab3777. (US20110311553).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format for improved compatibility with existing reagents assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))
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别名
- PAR2
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背景
- F2RL1, PAR-2, GPR11, Proteinase-activated receptor 2, Coagulation factor II receptor-like 1, G-protein coupled receptor 11, Thrombin receptor-like 1, Ab3777
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UniProt
- P55085
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome
抗原
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