Recombinant PD-1 抗体
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北京 101111
Quick Overview for Recombinant PD-1 抗体 (ABIN7543217)
抗原
See all PD-1 (PDCD1) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-PD-1 [29F.1A12], Rabbit IgG, kappa
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特异性
- This antibody recognizes an epitope on mouse PD-1.
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产品特性
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Original Species of Ab: Rat
Original Format of Ab: IgG2a
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纯化方法
- Protein A affinity purified
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免疫原
- The antibody was raised by immunizing rats with plasmid DNA containing PD-1.
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亚型
- IgG kappa
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anti-Programmed Cell Death 1 (PDCD1) (AA 201-288) antibody
PDCD1 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 1 (PDCD1) (AA 41-132) antibodyPDCD1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 1 (PDCD1) (AA 192-288) antibodyPDCD1 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 3B3D9 unconjugated
anti-Programmed Cell Death 1 (PDCD1) (AA 192-288) antibodyPDCD1 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 8G5B12 unconjugated
anti-Programmed Cell Death 1 (PDCD1) (AA 21-170) antibodyPDCD1 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 1D6F6 unconjugated
anti-Programmed Cell Death 1 (PDCD1) (AA 21-170) antibodyPDCD1 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 3E12A10 unconjugated
anti-Programmed Cell Death 1 (PDCD1) (AA 21-170) antibodyPDCD1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 1 (PDCD1) (AA 1-288) antibodyPDCD1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Programmed Cell Death 1 (PDCD1) antibodyPDCD1 适用: 人 IHC, ELISA, StM, Coat 宿主: 小鼠 Monoclonal PDCD1-2720 unconjugated
anti-Programmed Cell Death 1 (PDCD1) (AA 41-132) antibodyPDCD1 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C3 unconjugated
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应用备注
- This antibody was used for detection of PD-1 in CHO cells using flow cytometry. The ability of the antibody to block PD-1 interaction with PD-L1 or PD-L2 on CHO cells in an in vitro blocking assay was tested, the clone blocked PD-L1 Ig and PD-L2 Ig binding at high concentration, but only blocked 35 % of PD-L1 Ig or PD-L2 Ig binding at 10 μg/mL. This antibody was also used for immunohistochemical studies of various mouse tissues, such as heart (Liang et al, 2003, pmid: 14515254). In vivo anti-tumor effect in terms of level of tumor growth reduction was studied, a significant tumor growth reduction was observed in the tumors from mice treated with the antibody (Tobias et al, 2020, pmid: 32528470). The antibody was used for the detection of PD-1 in EL4 cells using flow cytometry. The antibody was used for cross-blocking with the unconjugated version of the same clones and it was reported to prevent PD-1 from interacting with PD-L1 (Polesso et al, 2021, pmid: 33684223). The antibody was used as a positive control for CT26 tumor model, a murine model of colon cancer (Kaumaya ey al., pmid:33117602).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
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别名
- PD-1
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背景
- CD279, Programmed cell death protein 1, Protein PD-1, mPD-1
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UniProt
- Q02242
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途径
- Cancer Immune Checkpoints
抗原
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