Recombinant FAP 抗体
Quick Overview for Recombinant FAP 抗体 (ABIN7543216)
抗原
See all FAP 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-FAP [3F2], Rabbit IgG, kappa
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特异性
- This antibody can bind human, mouse and cynomolgus monkey fibroblast activation protein.
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交叉反应
- Cynomolgus, 小鼠
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG2a
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纯化方法
- Protein A affinity purified
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亚型
- IgG kappa
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应用备注
- The original mouse antibody was used to check the expression levels of FAP on the surface of GM05389 lung fibroblasts using flow cytometry. The original mouse IgG1 version of this antibody binds human, mouse and cynomolgus monkey FAP with an avidity of 39 pM, 29 pM and 42 pM respectively. Binding of human IgG1 antibody 3F2 to human and murine FAP expressed on stably transfected HEK293 cells, human fibroblasts cell line GM05389 was measured by floy cytometry. The mouse IgG2a version of this antibody was also used for the immunohistochemical stainings of frozen tissue microarray (TMA) containing 10 invasive ductal carcinomas of the breast, 10 colorectal adenocarcinomas and 10 non-small cell lung cancers (US20200079873).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- FAP (Fibroblast Activation Protein, alpha (FAP))
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别名
- FAP
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背景
- Seprase, Fibroblast activation protein, Prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, FAPalpha, Fibroblast activation protein alpha, Gelatin degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, SIMP, Surface-expressed protease
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UniProt
- Q12884
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途径
- Tube Formation
抗原
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